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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Inhibition of STAT3 phosphorylation by sulforaphane reduces adhesion molecule expression in vascular endothelial cell
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Inhibition of STAT3 phosphorylation by sulforaphane reduces adhesion molecule expression in vascular endothelial cell

机译：萝卜硫烷对STAT3磷酸化的抑制作用降低血管内皮细胞黏附分子的表达

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摘要

Intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) play key roles in the initiation of vascular inflammation. In this study, we explored whether sulforaphane, a dietary phytochemical, can inhibit the expression of ICAM-1 and VCAM-1 in human umbilical vein endothelial cells (HUVEC) stimulated with lipopolysaccharide (LPS), and the mechanisms involved. Sulforaphane prevented the LPS-mediated increase in ICAM-1 and VCAM-1 expression, (P < 0.01) in HUVEC. Sulforaphane also prevented the LPS-mediated increase in the phosphorylation of signal transducer and activator of transcription 3 (STAT3) (P < 0.01). Stattic, a STAT3 inhibitor, reduced the LPS-induced expression of ICAM-1 and VCAM-1, and STAT3 phosphorylation (P < 0.01). STAT3 small interfering RNA treatment reduced the LPS-induced expression of ICAM-1, VCAM-1, and STAT3 (P < 0.01). Sulforaphane reduced LPS-mediated THP-1 monocyte adhesion to HUVEC (P < 0.01). In C57BL/6 mice, injection of LPS increased aortic ICAM-1 and VCAM-1 expression, and this effect was prevented by sulforaphane. These data provide insight into the mechanism through which sulforaphane partly reduces the expression of ICAM-1 and VCAM-1 on the vascular wall by inhibiting STAT3 phosphorylation.
机译：细胞间粘附分子1（ICAM-1）和血管细胞粘附分子1（VCAM-1）在引发血管炎症中起关键作用。在这项研究中，我们探讨了饮食植物化学物质萝卜硫素是否可以抑制脂多糖（LPS）刺激的人脐静脉内皮细胞（HUVEC）中ICAM-1和VCAM-1的表达，以及涉及的机制。萝卜硫素阻止HUVEC中LPS介导的ICAM-1和VCAM-1表达增加（P <0.01）。萝卜硫烷还阻止了LPS介导的信号转导子和转录激活子3（STAT3）的磷酸化增加（P <0.01）。 Stattic（一种STAT3抑制剂）可降低LPS诱导的ICAM-1和VCAM-1的表达以及STAT3磷酸化（P <0.01）。 STAT3小干扰RNA处理降低了LPS诱导的ICAM-1，VCAM-1和STAT3的表达（P <0.01）。萝卜硫烷降低了LPS介导的THP-1单核细胞对HUVEC的粘附（P <0.01）。在C57BL / 6小鼠中，注射LPS可增加主动脉ICAM-1和VCAM-1的表达，而萝卜硫烷可阻止这种作用。这些数据提供了对萝卜硫烷通过抑制STAT3磷酸化而部分减少ICAM-1和VCAM-1在血管壁上表达的机制的深入了解。

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