Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain

Mulholland V.; Schlenzig A.; MacAskill G. A.; Green S.

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Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain

机译：实时定量PCR检测试剂盒的开发，用于检测英国疫病菌Austrocedrae

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A TaqMan real-time PCR assay was developed for Phytophthora austrocedrae, an emerging pathogen causing severe damage to juniper in Britain. The primers amplified DNA of the target pathogen down to 1pg of extracted DNA, in both the presence and absence of host DNA, but did not amplify any of the non-target Phytophthora and fungal species tested. The assay provides a useful tool for screening juniper populations for the disease.

机译：TaqMan实时荧光定量PCR分析方法被开发用于疫霉，这是一种对英国杜松造成严重损害的新兴病原体。在存在和不存在宿主DNA的情况下，引物均将目标病原体的DNA扩增至提取的DNA的1pg，但未扩增所测试的任何非目标疫霉菌和真菌物种。该测定法为筛查杜松种群的疾病提供了有用的工具。

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《Forest Pathology》 |2013年第6期|共5页
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Mulholland V.; Schlenzig A.; MacAskill G. A.; Green S.;
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1. Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain [J] . Mulholland V., Schlenzig A., MacAskill G. A., Forest Pathology . 2013,第6期

机译：实时定量PCR检测试剂盒的开发，用于检测英国疫病菌Austrocedrae
2. Real-time quantitative PCR assays for evaluation of soybean varieties for resistance to the stem and root rot pathogen Phytophthora sojae [J] . Catal Mursel, Erler Fedai, Fulbright Dennis W., European Journal of Plant Pathology . 2013,第4期

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3. Selection of internal control genes for real-time quantitative RT-PCR assays in the oomycete plant pathogen Phytophthora parasitica [J] . Yan HZ, Liou RF Fungal Genetics and Biology . 2006,第6期

机译：卵菌植物病原菌疫霉菌实时定量RT-PCR检测的内部对照基因的选择
4. Development of Real-Time PCR Assays for the Quantitative Detection of CD81 Receptor Gene of Hepatitis C Virus in Tupaia Belangeri [C] . Feng, Yue, Feng, Yuemei, Bai, Jie, International Conference on Biomedical Engineering and Informatics;BMEI '09 . 2009

机译：实时PCR检测定量检测塔帕亚白热病中丙型肝炎病毒CD81受体基因的研究
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机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Development of a 5′ Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Cariogenic Dental Pathogens Streptococcus mutans and Streptococcus sobrinus [O] . Akihiro Yoshida, Nao Suzuki, Yoshio Nakano, 2003

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Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain

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