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首页> 外文期刊>Forest Pathology >Initial characterization of an unidentified Armillaria isolate from Serbia using LSU-IGS1 and TEF-1-alpha genes
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Initial characterization of an unidentified Armillaria isolate from Serbia using LSU-IGS1 and TEF-1-alpha genes

机译：使用LSU-IGS1和TEF-1-alpha基因初步鉴定来自塞尔维亚的未鉴定蜜环菌

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摘要

Armillaria species have a global distribution and play variable ecological roles, including causing root disease of diverse forest, ornamental and horticultural trees. Accurate identification of Armillaria species is critical to understand their distribution and ecological roles. This work focused on characterizing an unidentified Armillaria isolate from a Serbian forest using pairing, sequencing of the partial large subunit and intergenic spacer-1 regions of rDNA (LSU-IGS1) and the translation elongation factor-1 alpha gene (tef-1) genes, and phylogenetic analyses. Despite previously obtained LSU-IGS1 RFLP patterns that matched the newly described North American Armillaria altimontana, pairing tests and phylogenetic analyses of LSU-IGS1 and tef-1 sequences clearly demonstrate that the unidentified isolate is not A.altimontana. Based on LSU-IGS1, Armillaria gallica isolates were polyphyletic, and the Serbian isolate clustered with a subset of European A.gallica isolates within a well-supported clade (99%). Based on tef-1, the Serbian isolate appeared as a separate, well-supported clade (97%) that was basal to other poorly resolved, polyphyletic clades containing European A.gallica isolates. It is speculated that the unidentified Armillaria isolate from Serbia could represent an evolutionary ancestral state because of its separate, basal position compared with other clades comprising polyphyletic European A.gallica isolates. Alternatively, this unidentified Serbian isolate could represent an unusual hybrid because of its high-level sequence heterogeneity, represented by multiple two-nucleotide codes, within tef-1. Further characterization is needed to confirm the taxonomic status and ecological/evolutionary significance of this unique, unknown Armillaria isolate from Serbia.
机译：蜜环菌属物种具有全球分布，并发挥着可变的生态作用，包括引起各种森林，观赏和园艺树木的根系病害。准确识别蜜环菌物种对于了解其分布和生态作用至关重要。这项工作着眼于使用rDNA（LSU-IGS1）的部分大亚基和基因间隔区1区域以及翻译延伸因子1 alpha基因（tef-1）基因的配对，测序来表征塞尔维亚森林中未鉴定的蜜环菌分离株，以及系统发育分析。尽管先前获得的LSU-IGS1 RFLP模式与新近描述的北美蜜环菌（Armelaria altimontana）相匹配，但LSU-IGS1和tef-1序列的配对测试和系统发育分析清楚地表明，未鉴定的分离物不是拟南芥。基于LSU-IGS1，蜜环菌的分离株具有多系性，塞尔维亚分离株与欧洲gal曲霉的一部分分离株聚集在良好支撑的进化枝中（99％）。基于tef-1，塞尔维亚分离株表现为单独的，得到良好支持的进化枝（占97％），是其他解析度较差，含有欧洲A.gallica分离株的多系进化枝的基础。据推测，来自塞尔维亚的未鉴定的蜜环菌分离物可能代表了进化的祖先状态，因为与包含多系欧洲A.gallica分离物的其他进化枝相比，其分离的基础位置。另外，这种未知的塞尔维亚分离株可能代表一种不寻常的杂种，因为它的高水平序列异质性由tef-1内的多个两个核苷酸代码表示。需要进一步表征以确认这种独特的，未知的来自塞尔维亚的蜜环菌分离株的分类学地位和生态/进化意义。

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