NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-receptor interaction.

Giragossian C; Pellegrini M; Mierke DF

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NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-receptor interaction.

机译：CCK-8 / CCK1复合物的NMR研究支持膜与配体-受体相互作用的途径。

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The interaction of peptide ligands with their associated G-protein-coupled receptors has been examined by a number of different experimental approaches over the years. We have been developing an approach utilizing high-resolution NMR to determine the structural features of the peptide ligand, well-designed fragments of the receptor, and the ligand-receptor complexes formed upon titration of the peptide hormone. The results from these investigations provide evidence for a membrane-associated pathway for the initial interaction of peptide ligands with the receptor. Here, our results from the investigation of the interaction of CCK-8 with the CCK1 receptor are described. Our spectroscopic results clearly show that both CCK-8 and the regions of CCK1 with which it interacts are closely associated with the zwitterionic interface of the lipids utilized in our solution spectroscopic studies.

机译：多年来，肽配体与其相关的G蛋白偶联受体的相互作用已通过许多不同的实验方法进行了检验。我们一直在开发一种利用高分辨率NMR来确定肽配体，精心设计的受体片段以及在滴定肽激素后形成的配体-受体复合物的结构特征的方法。这些研究的结果为肽配体与受体的初始相互作用提供了膜相关途径的证据。在这里，我们的调查结果描述了CCK-8与CCK1受体的相互作用。我们的光谱结果清楚地表明，CCK-8和与其相互作用的CCK1区域都与我们在溶液光谱研究中使用的脂质的两性离子界面密切相关。

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《Canadian Journal of Physiology and Pharmacology》 |2002年第5期|共5页
作者
Giragossian C; Pellegrini M; Mierke DF;
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正文语种 eng
中图分类人体生理学;药理学;生理学;药学;
关键词
receptor; Sincalide; Ligands; Peptides; Nuclear Magnetic Resonance; 辛卡利特; 配体; 肽类; 核磁共振;

机译：receptor;Sincalide;Ligands;Peptides;Nuclear Magnetic Resonance;辛卡利特;配体;肽类;核磁共振;

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1. NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-receptor interaction. [J] . Giragossian C, Pellegrini M, Mierke DF Canadian Journal of Physiology and Pharmacology . 2002,第5期

机译：CCK-8 / CCK1复合物的NMR研究支持膜与配体-受体相互作用的途径。
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NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-receptor interaction.

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