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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >A fluorescent compound for glucose uptake measurements in isolated rat cardiomyocytes.
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A fluorescent compound for glucose uptake measurements in isolated rat cardiomyocytes.

机译：一种用于测量离体大鼠心肌细胞中葡萄糖摄取的荧光化合物。

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摘要

A focus of current diabetes research is the development of insulinomimetic compounds for oral treatment of diabetes and its associated cardiac complications. Screening compounds for their potential insulinomimetic effects usually involves the use of radioactive isotopes. The focus of this study was to investigate a nonradioactive fluorescent compound for its use in screening insulinomimetic compounds. The indicator 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) has been used by some workers to measure glucose uptake in Escherichia coli and Candida albicans. We propose that 2-NBDG will also be a suitable indicator for mammalian cell lines, in particular rat cardiomyocytes. We found that the indicator could give a reliable reproducible standard curve following appropriate dilution and is taken up by isolated cardiomyocytes. The insulinomimetic compounds vanadyl sulfate and sodium molybdate showed rates of glucose uptake similar to that of insulin. Furthermore, the rate of uptake measured for insulin using this technique (0.04 +/- 0.003 nmol x min(-1) x 10(6) cells(-1) is comparable with previous literature using 2-deoxyglucose uptake measurements on isolated myocytes (0.040 nmol x min(-1) x 10(6) cells(-1), demonstrating the validity of this fluorescent compound for glucose uptake studies.
机译：当前糖尿病研究的焦点是用于口服治疗糖尿病及其相关心脏并发症的胰岛素模拟化合物的开发。筛选化合物的潜在拟胰岛素作用通常涉及使用放射性同位素。这项研究的重点是研究一种用于筛选胰岛素模拟化合物的非放射性荧光化合物。某些工人已使用指示剂2-（N-（7-硝基苯-2-氧杂-1,3-二氮杂-4-基）氨基）-2-脱氧葡萄糖（2-NBDG）来测量大肠杆菌中的葡萄糖摄取和白色念珠菌。我们提出2-NBDG也将是哺乳动物细胞系，特别是大鼠心肌细胞的合适指示剂。我们发现，在适当稀释后，该指示剂可以给出可靠的可再现标准曲线，并被分离的心肌细胞吸收。胰岛素模拟化合物硫酸氧钒和钼酸钠显示出与胰岛素相似的葡萄糖摄取速率。此外，使用该技术测量的胰岛素摄取率（0.04 +/- 0.003 nmol x min（-1）x 10（6）cells（-1）与以前的文献中使用2-脱氧葡萄糖摄取测量的对分离的心肌细胞的摄取率相当（ 0.040 nmol x min（-1）x 10（6）细胞（-1），证明该荧光化合物对葡萄糖摄取研究的有效性。

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