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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Potential cytoprotection: antioxidant defence by caffeic acid phenethyl ester against free radical-induced damage of lipids, DNA, and proteins.
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Potential cytoprotection: antioxidant defence by caffeic acid phenethyl ester against free radical-induced damage of lipids, DNA, and proteins.

机译：潜在的细胞保护作用：咖啡酸苯乙酯的抗氧化防御作用，可抵抗自由基引起的脂质，DNA和蛋白质的破坏。

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摘要

Oxidative stress is considered to be a major cause of cellular injuries in a variety of chronic health problems, such as carcinogenesis and neurodegenerative disorders. Caffeic acid phenethyl ester (CAPE), derived from the propolis of honeybee hives, possesses a variety of biological and pharmacological properties including antioxidant and anticancer activity. In the present study, we focused on the diverse antioxidative functionalities of CAPE and its related polyphenolic acid esters on cellular macromolecules in vitro. The effects on human erythrocyte membrane ghost lipid peroxidation, plasmid pBR322 DNA, and protein damage initiated by the water-soluble initiator 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH) and hydrogen peroxide (H(2)O(2)) were monitored by formation of hydroperoxides and by DNA nicking assay, single-cell alkaline electrophoresis, and SDS-polyacrylamide gel electrophoresis. Our results showed that CAPE and its related polyphenolic acid esters elicited remarkable inhibitory effects on erythrocyte membrane lipid peroxidation, cellular DNA strand breakage, and protein fragmentation. The results suggest that CAPE is a potent exogenous cytoprotective and antigenotoxic agent against cell oxidative damage that could be used as a template for designing novel drugs to combat diseases induced by oxidative stress components, such as various types of cancer.
机译：氧化应激被认为是各种慢性健康问题（如致癌作用和神经退行性疾病）中细胞损伤的主要原因。源自蜂巢蜂胶的咖啡酸苯乙酯（CAPE）具有多种生物学和药理特性，包括抗氧化剂和抗癌活性。在本研究中，我们集中于CAPE及其相关的多酚酸酯对细胞大分子的体外抗氧化功能。水溶性引发剂2,2'-偶氮二（2-ami基丙烷）盐酸盐（AAPH）和过氧化氢（H（2）O（2）对人红细胞膜鬼脂质过氧化，质粒pBR322 DNA和蛋白质损伤的影响））通过过氧化氢的形成，DNA切口测定，单细胞碱性电泳和SDS-聚丙烯酰胺凝胶电泳进行监测。我们的结果表明，CAPE及其相关的多酚酸酯对红细胞膜脂质过氧化，细胞DNA链断裂和蛋白质片段化具有明显的抑制作用。结果表明，CAPE是一种有效的针对细胞氧化损伤的外源性细胞保护和抗原毒性药物，可以用作设计新型药物以对抗由氧化应激成分引起的疾病（例如各种类型的癌症）的模板。

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