• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Glutaminergic and adrenergic receptors expressed on adult guinea pig Schwann cells in vitro.
【24h】

Glutaminergic and adrenergic receptors expressed on adult guinea pig Schwann cells in vitro.

机译：谷氨酰胺和肾上腺素能受体在成年豚鼠雪旺细胞上体外表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have investigated the responsiveness of adult guinea pig Schwann cells to a range of neuroligands, using ratiometric calcium imaging. The majority of cells responded to ATP (90 +/- 4%), adrenaline (57 +/- 5%), and noradrenaline (61 +/- 5%), as well as glutamate (60 +/- 5%). The number of cells responding to glutamate increased significantly (90 +/- 4%; p < 0.01) when the cells were grown in excitatory amino acid (EAA) free medium, indicating EAA-induced downregulation. Only a small number of cells (9 +/- 2%) responded to acetylcholine. Agonist and antagonist experiments show that these adult Schwann cells predominantly express ionotropic glutaminergic receptors (N-methyl-D-aspartate (NMDA), alpha-amino-3-hydroxy-5-methyl-4-isooxazolepropionic acid (AMPA), and kainate) as well as alpha1-, alpha2-, and beta-adrenoreceptors. We conclude that Schwann cells derived from adult guinea pigs express a variety of neuroligand receptors when established in culture and are particularly rich in glutamate receptors. This probably reflects a de-differentiated state important to development and regeneration.
机译：我们已经使用比例钙成像技术研究了成年豚鼠雪旺氏细胞对一系列神经配体的反应性。大部分细胞对ATP（90 +/- 4％），肾上腺素（57 +/- 5％）和去甲肾上腺素（61 +/- 5％）以及谷氨酸（60 +/- 5％）有反应。当细胞在无兴奋性氨基酸（EAA）的培养基中生长时，对谷氨酸有反应的细胞数量显着增加（90 +/- 4％; p <0.01），表明EAA诱导的下调。只有少数细胞（9 +/- 2％）对乙酰胆碱有反应。激动剂和拮抗剂实验表明，这些成年的雪旺细胞主要表达离子型谷氨酰胺受体（N-甲基-D-天冬氨酸（NMDA），α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）和海藻酸盐）以及alpha1，alpha2和β肾上腺素受体。我们得出的结论是，成年豚鼠衍生的雪旺细胞在培养物中表达时会表达多种神经配体受体，并且谷氨酸受体特别丰富。这可能反映了对发育和再生很重要的去分化状态。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号