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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Time-related effect of GnRH on histone H1 kinase activity in the goldfish follicle-enclosed oocyte.
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Time-related effect of GnRH on histone H1 kinase activity in the goldfish follicle-enclosed oocyte.

机译：GnRH对金鱼卵泡封闭卵母细胞中组蛋白H1激酶活性的时间相关影响。

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摘要

The level of cyclin B-associated cdc2 kinase, a component of maturation promoting factor (MPF), is known to be high during metaphase of the meiotic maturation of oocytes. The time-related action of gonadotropin-releasing hormones (GnRH) on histone H1 kinase activity (known to reflect cdc2 kinase activity) was investigated in vitro in follicle-enclosed goldfish oocytes. Germinal vesicle breakdown (GVBD) and testosterone production were also investigated in the same follicle-enclosed goldfish oocytes to determine the temporal relationship between GnRH-induced histone H1 kinase activity and the reinitiation of meiosis and steroidogenesis. Treatments with gonadotropin (GTH) or GnRH stimulated the histone H1 kinase activity to the same maximum level. However, sGnRH- and cGnRH-II-induced histone H1 kinase activity were significantly higher compared with controls after 2 hours of treatment, whereas the GTH-induced increase became significantly higher after 6-8 hours of incubation. Overall, the results demonstrate a close temporal relationship between GVBD response and histone H1 kinase activity induced by GTH and sGnRH-cGnRH-II.
机译：已知与周期蛋白B相关的cdc2激酶（成熟促进因子（MPF）的组成部分）的水平在卵母细胞减数分裂成熟的中期很高。在卵泡封闭的金鱼卵母细胞中体外研究了促性腺激素释放激素（GnRH）对组蛋白H1激酶活性（已知反映cdc2激酶活性）的时间相关作用。还研究了在同一卵泡封闭的金鱼卵母细胞中的胚泡破裂（GVBD）和睾丸激素的产生，以确定GnRH诱导的组蛋白H1激酶活性与减数分裂和类固醇生成重新启动之间的时间关系。促性腺激素（GTH）或GnRH的治疗将组蛋白H1激酶活性刺激到相同的最大水平。然而，在处理2小时后，sGnRH和cGnRH-II诱导的组蛋白H1激酶活性明显高于对照组，而在孵育6-8小时后，GTH诱导的组蛋白H1激酶活性显着更高。总体而言，结果表明GVBD应答与GTH和sGnRH-cGnRH-II诱导的组蛋白H1激酶活性之间存在紧密的时间关系。

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