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首页> 外文期刊>Food Science and Technology International >Modeling the influence of pH, temperature and culture medium composition on the kinetics of growth and cysteine proteinase production by Micrococcus sp. INIA 528 in batch culture
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Modeling the influence of pH, temperature and culture medium composition on the kinetics of growth and cysteine proteinase production by Micrococcus sp. INIA 528 in batch culture

机译：模拟pH，温度和培养基组成对Micrococcus sp。生长和半胱氨酸蛋白酶产生动力学的影响。分批培养的INIA 528

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摘要

Kinetics of biomass and cysteine proteinase formation during growth of Micrococcus sp. INIA 528 in batch cultivation were studied. Modeling of cultivation data was carried out using an unstructured model. The logistic equation was found to be adequate to describe microbial growth. Production of cysteine proteinase was described by means of an equation including biomass and substrate concentrations. An overall model based on an equation of substrate consumption dependent on biomass formation and proteinase production was postulated. The kinetic parameters (specific growth rate, maximum biomass, proteinase production rate and proteinase formation yield) were obtained taking into account the influence of pH (6.0-8.0), temperature (28-41 deg C) and the addition of different supplements to the basal culture medium [yeast extract, MgSO_4, glucose, K_2HPO_4 and (NH_4)_2SO_4]. Kinetic parameters were calculated by fitting experimental data by a nonlinear technique using the algorithm of Marquardt coupledto a fourth-order Runge-Kutta subroutine.
机译：微球菌生长过程中生物量和半胱氨酸蛋白酶形成的动力学。研究了INIA 528的分批栽培。培养数据的建模是使用非结构化模型进行的。发现逻辑方程足以描述微生物的生长。半胱氨酸蛋白酶的产生通过包括生物量和底物浓度的方程式来描述。假定了一个基于底物消耗方程的整体模型，该方程取决于生物量的形成和蛋白酶的产生。考虑到pH值（6.0-8.0），温度（28-41℃）的影响以及向其中添加不同补品的影响，获得了动力学参数（比生长速率，最大生物量，蛋白酶产生速率和蛋白酶形成产率）。基础培养基[酵母提取物，MgSO_4，葡萄糖，K_2HPO_4和（NH_4）_2SO_4]。通过使用Marquardt算法和四阶Runge-Kutta子程序耦合的非线性技术，通过拟合实验数据来计算动力学参数。

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