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首页> 外文期刊>Gene expression >Beta-catenin associates with human parainfluenza virus type 3 ribonucleoprotein complex and activates transcription of viral genome RNA in vitro.
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Beta-catenin associates with human parainfluenza virus type 3 ribonucleoprotein complex and activates transcription of viral genome RNA in vitro.

机译：Beta-catenin与人类副流感病毒3型核糖核蛋白复合物缔合，并在体外激活病毒基因组RNA的转录。

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摘要

Several studies have indicated that human parainfluenza virus type 3 (HPIV-3) requires polymeric actin for transcription of its genome RNA in vitro and in vivo. In the current study, we have identified beta-catenin, an actin-bound protein, as one of the transcriptional activators for HPIV-3 genome RNA. Beta-catenin was packaged within the purified HPIV-3 virions and was associated with the HPIV-3 ribonucleoproteins (RNP) from infected cells. Moreover, purified beta-catenin interacted with bacterially expressed HPIV-3 nucleocapsid protein (N) and phosphoprotein (P) fused to glutathione S-transferase (GST). Double-labeled immunofluorescent confocal microscopic analysis revealed colocalization of beta-catenin with HPIV-3 RNP at cell periphery in infected cells. The HPIV-3 RNP-associated beta-catenin functioned as a transactivator of HPIV-3 genome, because purified beta-catenin stimulated transcription of viral RNP in an in vitro transcription assay. These results demonstrate that beta-catenin, a multifunctional protein that is involved in cell-cell adhesion and embryogenesis, acts as one of the transcriptional activators of HPIV-3 genome RNA.
机译：多项研究表明，人类副流感病毒3型（HPIV-3）需要聚合肌动蛋白才能在体内和体外转录其基因组RNA。在当前的研究中，我们确定了β-catenin（一种肌动蛋白结合蛋白）是HPIV-3基因组RNA的转录激活因子之一。 β-catenin包装在纯化的HPIV-3病毒体中，并与来自感染细胞的HPIV-3核糖核蛋白（RNP）相关。此外，纯化的β-catenin与细菌表达的HPIV-3核衣壳蛋白（N）和与谷胱甘肽S-转移酶（GST）融合的磷蛋白（P）相互作用。双重标记的免疫荧光共聚焦显微镜分析显示，β-catenin与HPIV-3 RNP在感染细胞的细胞外围共定位。 HPIV-3 RNP相关的β-catenin可以作为HPIV-3基因组的反式激活因子，因为在体外转录测定中纯化的β-catenin刺激了病毒RNP的转录。这些结果表明，β-catenin是一种参与细胞-细胞粘附和胚胎发生的多功能蛋白，是HPIV-3基因组RNA的转录激活因子之一。

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