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首页> 外文期刊>Gene expression >Tissue, species, and environmental differences in absolute quantities of murine mRNAs coding for alpha, mu, omega, pi, and theta glutathione S-transferases.
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Tissue, species, and environmental differences in absolute quantities of murine mRNAs coding for alpha, mu, omega, pi, and theta glutathione S-transferases.

机译：编码α，mu，omega，pi和theta谷胱甘肽S-转移酶的鼠mRNA绝对数量的组织，种类和环境差异。

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摘要

This article reports the first absolute quantitative analysis of expression patterns of murine transcripts (Gsta1/2, Gsta3, Gsta4, Gstm1, Gstm2, Gstm3, Gsto1, Gstp1/2, Gstt1, Gstt2) coding for most glutathione S-transferases (GSTs) of alpha, mu, omega, pi, and theta classes. We examine how the steady-state numbers of transcripts are modulated in association with: three animal organs (liver, kidney, and lung) where extensive detoxification occurs; two species (Mus musculus and Mus spretus) representing common laboratory and aboriginal mice; and two genetic and animal living conditions (wild-derived inbred animals and free-living mice). Moreover, quantitations performed examine how the pulmonary steady-state Gst mRNA amounts are affected in M. musculus by paraquat (a superoxide generator), and in M. spretus by dwelling at a polluted area. The results point to complex tissue-, species-, and life condition-dependent expression of the investigated transcripts. Among others, they show: i) the ubiquity of most transcripts, except Gstm3 mRNA that was virtually absent or at very low amounts (< or = 0.001 molecules/pg) in kidney and lung of M. spretus; ii) unique expression profiles for each transcript and mouse organ examined; iii) outstanding species-specific differences in basal amounts of most Gst mRNAs, this effect being most apparent in the case of Gsta1/2, Gsta3, Gstm2, Gsto1, Gstt1, and Gstt2; iv) paraquat-induced upregulation of most Gst mRNAs, with the notable exception of those coding for theta class GSTs; v) a tendency for mice dwelling at a wildlife reserve of having lower and more homogeneous Gsta3 mRNA levels than those collected in an anthropogenic environment.
机译：本文报道了编码大多数谷胱甘肽S-转移酶（GST）的鼠类转录本（Gsta1 / 2，Gsta3，Gsta4，Gstm1，Gstm2，Gstm3，Gsto1，Gstp1 / 2，Gstt1，Gstt2）的表达模式的首次绝对定量分析。 alpha，mu，omega，pi和theta类。我们研究与以下相关的转录物的稳态数目如何调节：发生排毒的三个动物器官（肝，肾和肺）;以及代表普通实验室鼠和原住鼠的两种物种（小家鼠和小家鼠）;以及两种遗传和动物的生存条件（野生近交动物和自由生活的小鼠）。此外，进行的定量分析检查百草枯（一种超氧化物产生剂）如何对小家鼠支原体和寄居于支原体的肺稳态Gst mRNA量产生影响。结果指向研究的转录本的复杂的组织，物种和生活条件依赖性表达。除其他外，它们显示：i）大多数转录物的普遍存在，除了Gstm3 mRNA在猪分枝杆菌的肾脏和肺中实际上不存在或含量极低（<或= 0.001分子/ pg）之外; ii）每个转录本和小鼠器官的独特表达谱; iii）大多数Gst mRNA的基础量存在显着的物种特异性差异，这种效应在Gsta1 / 2，Gsta3，Gstm2，Gsto1，Gstt1和Gstt2的情况下最为明显; iv）百草枯引起的大多数Gst mRNA的上调，但编码theta类GST的除外。 v）居住在野生动植物保护区的小鼠的Gsta3 mRNA水平要比在人为环境中采集的小鼠更低和更均一的趋势。

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