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首页> 外文期刊>Gene expression >Stable expression of FoxA1 promotes pluripotent P19 embryonal carcinoma cells to be neural stem-like cells
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Stable expression of FoxA1 promotes pluripotent P19 embryonal carcinoma cells to be neural stem-like cells

机译：FoxA1的稳定表达促进多能P19胚胎癌细胞成为神经干样细胞

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摘要

FoxA1 belongs to the fork head/winged-helix transcription factor family and participates in stimulating neuronal differentiation of pluripotent stem cells at early stages. To explore the biological roles of FoxA1 during this process, the stable expression of a GFP-FoxA1 fusion protein was established in P19 pluripotent embryonal carcinoma cells. Although they still express pluripotency-related transcription factors such as Oct4, Nanog, and Sox2, the generated P19 GFPFoxA1 cells exhibited a decreased activity of alkaline phosphatase and an increased expression of SSEA-3 compared with P19 cells. Elevated levels of nestin expression and prominin-1 + populations were observed in P19 GFPFoxA1 cells, implicating that the stable expression of FoxA1 promoted P19 cells to gain partial characteristics of neural stem cells. Furthermore, the promoter of nestin was confirmed to be bound and activated by FoxA1 directly. The expression of neuron-specific marker tubulin βIII also existed in P19 GFPFoxA1 cells. P19 GFPFoxA1 cells showed an earlier onset of differentiation during RA-induced neuronal differentiation, evidenced by a more rapid change on the Nanog decrease and the tubulin βIII increase. Thus, overexpression of FoxA1 alone may promote pluripotent P19 cells to become neural stem-like cells.
机译：FoxA1属于叉头/有翼螺旋转录因子家族，并在早期参与刺激多能干细胞的神经元分化。为了探索FoxA1在此过程中的生物学作用，在P19多能胚胎癌细胞中建立了GFP-FoxA1融合蛋白的稳定表达。尽管它们仍表达多能性相关的转录因子，例如Oct4，Nanog和Sox2，但与P19细胞相比，生成的P19 GFPFoxA1细胞显示出碱性磷酸酶活性降低和SSEA-3表达增加。在P19 GFPFoxA1细胞中观察到了巢蛋白表达水平和prominin-1 +群体的升高，这表明FoxA1的稳定表达促进了P19细胞获得神经干细胞的部分特征。此外，证实巢蛋白的启动子被FoxA1直接结合和激活。神经元特异性标记微管蛋白βIII的表达也存在于P19 GFPFoxA1细胞中。 P19 GFPFoxA1细胞在RA诱导的神经元分化过程中显示出较早的分化开始，这由Nanog降低和微管蛋白βIII升高的更快变化所证明。因此，仅FoxA1的过表达可能促进多能P19细胞成为神经干样细胞。

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