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首页> 外文期刊>Gene expression >Expression patterns of ubiquitin conjugating enzyme UbcM2 during mouse embryonic development
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Expression patterns of ubiquitin conjugating enzyme UbcM2 during mouse embryonic development

机译：泛素结合酶UbcM2在小鼠胚胎发育过程中的表达模式

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摘要

Ubiquitin conjugating enzyme UbcM2 (Ubiquitin-conjugating enzymes from Mice, the number reveals the identification order) has been implicated in many critical processes, such like growth-inhibiting, mediating cell proliferation and regulation of some transcription factor, but the expression profile during mouse embryo development remains unclear. Hereby, during mid-later embryonic stage, the expression patterns of UbcM2 were examined using in situ hybridization and quantitative real-time PCR (qRT-PCR). The signals were significantly intense in central nervous system and skeletal system, weak in tongue, heart, lung, liver, and kidney. In the central nervous system, UbcM2 was principally expressed in thalamus, external germinal layer of cerebellum (EGL), mitral cell layer of olfactory bulb, hippocampus, marginal zone and ventricular zone of cerebral cortex, and spinal cord. In the skeletal system, UbcM2 was primarily expressed in proliferating cartilage. Furthermore, qRT-PCR analysis displayed that the expression of UbcM2 was ubiquitous at E15.5, most prominent in brain, weaker in lung liver and kidney, accompanied by the lowest level in tongue and heart. During brain development, the expression level of UbcM2 first ascended and then decreased from E12.5 to E18.5, the peak of which sustained starting at E14.5 until E16.5. Together, these results suggest that UbcM2 may play potential roles in the development of mouse diverse tissues and organs, particularly in the development of brain and skeleton.
机译：泛素结合酶UbcM2（来自小鼠的泛素结合酶，数字揭示了鉴定顺序）已牵涉到许多关键过程，例如生长抑制，介导细胞增殖和某些转录因子的调控，但在小鼠胚胎期的表达谱发展尚不清楚。因此，在后期后期，使用原位杂交和定量实时PCR（qRT-PCR）检测UbcM2的表达模式。信号在中枢神经系统和骨骼系统中明显增强，在舌头，心脏，肺，肝和肾脏中则弱。在中枢神经系统中，UbcM2主要在丘脑，小脑外生发层（EGL），嗅球的二尖细胞层，海马，大脑皮质的边缘区和心室区以及脊髓中表达。在骨骼系统中，UbcM2主要在增殖软骨中表达。此外，qRT-PCR分析显示，UbcM2的表达在E15.5处普遍存在，在脑中最突出，在肺肝和肾脏中较弱，在舌头和心脏中最低。在大脑发育过程中，UbcM2的表达水平先升后降，从E12.5降至E18.5，其峰值从E14.5持续到E16.5。总之，这些结果表明，UbcM2可能在小鼠多种组织和器官的发育中，特别是在大脑和骨骼的发育中发挥潜在作用。

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