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首页> 外文期刊>Gene expression >Differential expression of distinct surface markers in early endothelial progenitor cells and monocyte-derived macrophages
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Differential expression of distinct surface markers in early endothelial progenitor cells and monocyte-derived macrophages

机译：早期内皮祖细胞和单核细胞衍生的巨噬细胞中不同表面标志物的差异表达

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Bone marrow-derived endothelial progenitor cells (EPCs) play a fundamental role in postnatal angiogenesis. Currently, EPCs are defined as early and late EPCs based on their biological properties and their time of appearance during in vitro culture. Reports have shown that early EPCs share common properties and surface markers with adherent blood cells, especially CD14+ monocytes. Distinguishing early EPCs from circulating monocytes or monocyte-derived macrophages (MDMs) is therefore crucial to obtaining pure endothelial populations before they can be applied as part of clinical therapies. We compared the gene expression profiles of early EPCs, blood cells (including peripheral blood mononuclear cells, monocytes, and MDMs), and various endothelial lineage cells (including mature endothelial cells, late EPCs, and CD133+ stem cells). We found that early EPCs expressed an mRNA profile that showed the greatest similarity to MDMs than any other cell type tested. The functional significance of this molecular profiling data was explored by Gene Ontology database search. Novel plasma membrane genes that might potentially be novel isolation biomarkers were also pinpointed. Specifically, expression of CLEC5A was high in MDMs, whereas early EPCs expressed abundant SIGLEC8 and KCNE1. These detailed mRNA expression profiles and the identified functional modules will help to develop novel cell isolation approaches that will allow EPCs to be purified; these can then be used to target cardiovascular disease, tumor angiogenesis, and various ischemia-related diseases.
机译：骨髓来源的内皮祖细胞（EPC）在产后血管生成中起着基本作用。目前，根据EPC的生物学特性和在体外培养过程中出现的时间，它们被定义为早期和晚期EPC。报道表明，早期的EPC与粘附的血细胞，尤其是CD14 +单核细胞具有共同的特性和表面标记。因此，从循环单核细胞或单核细胞衍生的巨噬细胞（MDM）中区分出早期EPC，对于在将其用作临床治疗的一部分之前，获得纯内皮细胞至关重要。我们比较了早期EPC，血细胞（包括外周血单核细胞，单核细胞和MDM）和各种内皮细胞（包括成熟内皮细胞，晚期EPC和CD133 +干细胞）的基因表达谱。我们发现，早期的EPC表达的mRNA谱图与MDM的相似性比其他任何测试的细胞类型都高。通过基因本体数据库搜索探索了这种分子谱数据的功能意义。还指出了可能是新型分离生物标志物的新型质膜基因。具体而言，CLEC5A的表达在MDM中较高，而早期的EPC则表达丰富的SIGLEC8和KCNE1。这些详细的mRNA表达谱和已鉴定的功能模块将有助于开发新颖的细胞分离方法，从而使EPC得以纯化。然后可以将这些用于靶向心血管疾病，肿瘤血管生成和各种缺血相关疾病。

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