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首页> 外文期刊>Fish & Shellfish Immunology >Cloning and expression analysis of Atlantic halibut (Hippoglossus hippoglossus) CD3 genes
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Cloning and expression analysis of Atlantic halibut (Hippoglossus hippoglossus) CD3 genes

机译：大西洋比目鱼（Hippoglossus hippoglossus）CD3基因的克隆和表达分析

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The CD3 complex is in higher vertebrates shown to be important for the activation of T-cells. The T-cell system in fish is believed to be similar to that in higher vertebrates, and the CD3 chains could therefore be an important marker for identification of T-cells in fish. Here, we report the cDNA and corresponding gene sequence of Atlantic halibut (Hippoglossus hippoglossus) CD3 gamma delta , CD3 approximately equal to , and CD3 direct sum chains, and the tissue-specific expression pattern of CD3 and T- cell receptor (TCR) genes. Important structural characteristics defining the CD3 genes seemed to be conserved in the halibut CD3 chains, such as a signal peptide, an extracellular region, a transmembrane helix having a negatively charged residue, and an ITAM bearing cytoplasmic tail. The extracellular domain of halibut CD3 gamma delta and CD3 approximately equal to included two cysteines presumably involved in Ig-fold stabilisation and the CxxCxE motif important for dimerization. A spliced variant of CD3 approximately equal to was identified, lacking the Ig-fold, but with the CxxCxE motif intact. The real time RT-PCR analysis revealed a highly similar expression pattern of the CD3 genes and the TCR alpha and TCR beta genes, indicating that the functional relationship between the TCR and the CD3 genes are preserved in teleosts.
机译：CD3复合体位于高等脊椎动物中，被证明对激活T细胞很重要。鱼类中的T细胞系统被认为与高等脊椎动物中的T细胞系统相似，因此CD3链可能成为鉴定鱼类T细胞的重要标志。在这里，我们报告大西洋大比目鱼（Hippoglossus hippoglossus）CD3γδ的cDNA和相应的基因序列，大约等于CD3和CD3直接和链，以及CD3和T细胞受体（TCR）基因的组织特异性表达模式。定义CD3基因的重要结构特征似乎在大比目鱼CD3链中是保守的，例如信号肽，细胞外区域，带有带负电荷残基的跨膜螺旋和带有ITAM的胞质尾巴。大比目鱼CD3γδ和CD3的胞外域大约等于，包括两个可能参与Ig折叠稳定的半胱氨酸和对二聚化很重要的CxxCxE基序。鉴定出CD3的剪接变体约等于，其缺乏Ig-折叠，但具有完整的CxxCxE基序。实时RT-PCR分析显示CD3基因与TCR alpha和TCR beta基因的表达模式极为相似，表明TCR和CD3基因之间的功能关系保留在硬骨鱼中。

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