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首页> 外文期刊>Fish & Shellfish Immunology >Dietary administration of microalgae Navicula sp affects immune status and gene expression of gilthead seabream (Sparus aurata)
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Dietary administration of microalgae Navicula sp affects immune status and gene expression of gilthead seabream (Sparus aurata)

机译：饮食管理微藻Navicula sp会影响金头鲷（Sparus aurata）的免疫状态和基因表达

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摘要

Effects of silage microalgae enriched with a probiotic and lyophilized microalgae were evaluated on main immune parameters and different gene expression of gilthead seabream (Sparus aurata L). A total of 60 seabream were grouped into 3 treatment diets which were a control diet (commercial diet) without microalgae (C), commercial diet supplemented with silage microalgae Navicula sp. plus Lactobacillus sakei 5-4 (10(6) CFU g(-1)) (SM), and commercial diet supplemented with lyophilized microalgae (LM) for 4 weeks. Generally, the results showed a significant increase in the immune parameters, principally in leucocyte peroxidase, phagocytosis and complement activities in fish fed with SM diet compared to control group. About the gene expression in head-kidney, transcript levels (Interleukin-8, Interleukin-1 beta and beta-defensin) were upregulated in fish fed with SM after 4 weeks of treatments. However, the gene expression was upregulated in intestine from fish fed with LM with significant difference in transferrin and cyclooxygenase 2 gene at 2 weeks, and in occludin, transferrin, interleukin-8 and interleukin-1 beta at 4 weeks. Finally, about the digestive enzymes, LM diet caused an upregulated of alpha-amylase and alkaline phosphatase genes at 2 weeks; however SM diet caused an upregulated trypsin gene at 4 weeks. SM diet a higher enhancing effect on gilthead seabream immune parameters than that observed when using LM. Furthermore, dietary administration of microalgae Navicula sp. provokes upregulation of several genes in the gut that correlates with slight inflammation. Further studies are needed to know if this diatom could be useful for administering as diet supplement for farmed fish. (C) 2013 Elsevier Ltd. All rights reserved.
机译：评估了富含益生菌和冻干微藻的青贮微藻对主要免疫参数和银头鲷（Sparus aurata L）不同基因表达的影响。将总共​​60只鲷鱼分为3种治疗饮食，分别是不含微藻类（C）的对照饮食（商业饮食），补充有青贮饲料微藻Navicula sp的商业饮食。再加上清酒乳杆菌5-4（10（6）CFU g（-1））（SM）和商业饮食，并补充冻干微藻（LM），持续4周。通常，结果显示，与对照组相比，SM饲料喂养的鱼的免疫参数显着增加，主要是白细胞过氧化物酶，吞噬作用和补体活性。关于头部肾脏中的基因表达，处理4周后，喂有SM的鱼的转录本水平（白细胞介素8，白细胞介素1β和β防御素）上调。然而，该基因的表达在用LM喂养的鱼的肠中在2周时在转铁蛋白和环氧合酶2基因中有显着差异，而在4周时在闭合蛋白，转铁蛋白，白介素8和白介素-1β中有显着差异。最后，关于消化酶，LM饮食在2周时引起了α-淀粉酶和碱性磷酸酶基因的上调。然而，SM饮食在4周时导致胰蛋白酶基因上调。 SM饮食比使用LM饮食对金头鲷免疫参数的增强作用更高。此外，饮食管理的微藻Navicula sp。引起肠道中与轻微炎症相关的几个基因的上调。需要进一步的研究来了解这种硅藻是否可以作为养殖鱼类的饮食补充剂使用。 （C）2013 Elsevier Ltd.保留所有权利。

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