...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish & Shellfish Immunology >A novel C-type lectin from crab Eriocheir sinensis functions as pattern recognition receptor enhancing cellular encapsulation.
【24h】

A novel C-type lectin from crab Eriocheir sinensis functions as pattern recognition receptor enhancing cellular encapsulation.

机译：一种来自中华绒螯蟹的新型C型凝集素，可作为模式识别受体，增强细胞的包裹性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

C-type lectins are a large family of Ca2+-dependent carbohydrate binding proteins which play crucial roles to recognize and eliminate pathogens in innate immunity. In the present study, a novel C-type lectin was identified from Eriocheir sinensis (designated as EsCTL). The full-length cDNA of EsCTL was of 789 bp with an open reading frame of 468 bp encoding a polypeptide of 156 amino acids with a signal sequence and single carbohydrate-recognition domain (CRD). The potential tertiary structure of the CRD adopted a typical double-loop structure with Ca2+-binding site 2 in the long loop region and two conserved disulfide bridges at the bases of the loops. An EPQ motif to determine carbohydrate binding specificity was identified in the CRD of EsCTL. The mRNA transcripts of EsCTL were mainly detected in hepatopancreas and its relative expression level in hemocytes was significantly up-regulated after the challenges of Vibrio anguillarum (P<0.05) and Pichia pastoris (P<0.05). The recombinant EsCTL protein (rEsCTL) could bind different PAMPs, including LPS, PGN, beta -glucan, and polyI:C; and also bind various microorganisms including three Gram-positive bacteria, three Gram-negative bacteria and two yeasts. Moreover, rEsCTL could significantly enhance the in vitro encapsulation of crab hemocytes. All these results suggested that EsCTL functioned as an important PRR involved in immune defense against invading pathogen in crab.
机译：C型凝集素是Ca 2 + 依赖的碳水化合物结合蛋白的一大家族，在识别和消除先天性免疫中的病原体中起着至关重要的作用。在本研究中，从中华绒螯蟹（称为EsCTL）中鉴定出一种新型的C型凝集素。 EsCTL的全长cDNA为789 bp，开放阅读框为468 bp，编码156个氨基酸的多肽，并具有信号序列和单个碳水化合物识别域（CRD）。 CRD的潜在三级结构采用典型的双环结构，在长环区域具有Ca 2 + 结合位点2，在环的底部具有两个保守的二硫键。在EsCTL的CRD中鉴定了一个可确定碳水化合物结合特异性的EPQ基序。 EsCTL的mRNA转录物主要在肝胰腺中检出，在鳗弧菌（P <0.05）和巴斯德毕赤酵母（P <0.05）的攻击下，其在血细胞中的相对表达水平显着上调。重组EsCTL蛋白（rEsCTL）可以结合不同的PAMP，包括LPS，PGN，β-葡聚糖和polyI：C。并结合多种微生物，包括三种革兰氏阳性菌，三种革兰氏阴性菌和两种酵母。此外，rEsCTL可以显着增强蟹血细胞的体外封装。所有这些结果表明，EsCTL作为重要的PRR，参与了针对蟹的入侵病原体的免疫防御。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号