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首页> 外文期刊>Fish & Shellfish Immunology >A comparative study: In vitro effects of EPA and DHA on immune functions of head-kidney macrophages isolated from large yellow croaker (Larmichthys crocea)
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A comparative study: In vitro effects of EPA and DHA on immune functions of head-kidney macrophages isolated from large yellow croaker (Larmichthys crocea)

机译：一项比较研究：EPA和DHA对从大黄鱼（Larmichthys crocea）分离出的头部肾脏巨噬细胞免疫功能的体外作用

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Comparative effects of different concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on immune responses of head-kidney macrophages isolated from large yellow croaker were studied in vitro. After exposing to serum-free medium for 1 day, cultured cells were incubated in medium supplemented with graded levels of EPA or DHA (0, 5, 25, 100, 200 and 1000 mu M, respectively) in the form of fatty acid bovine serum albumin (FA-BSA) complex for 12 h, 24 h and 36 h, respectively. Control samples were incubated in the absence of EPA or DHA (2% bovine serum albumin, BSA). Following stimulation, cell viability, lipid peroxidation, secretary phopholipase A(2) (sPLA(2)) and prostaglandin E-2 (PGE(2)) production as well as some immune parameters including phagocytosis, respiratory burst activity and interleukin 1 beta (IL-1 beta) production were determined. Results showed that EPA and DHA affected cell viability in dose-dependent and time-dependent manners. In particular, cell viability was significantly decreased after 24 h and 36 h incubation with 1000 mu M EPA or DHA (P < 0.05). Higher levels of EPA (200 and 1000 mu M) caused a significant increase in the production of malondialdehyde (MDA) (P < 0.05), while DHA did not significantly affect the MDA production. EPA significantly increased the intracellular superoxide anion synthesis which, on the contrary, was significantly reduced by DHA. Phagocytosis percentage (PP) values were significantly higher in treatments with 5 mu M DHA (P < 0.05), but significantly decreased by 200 and 1000 mu M EPA and DHA compared to the control group (P < 0.05). Decreased PGE2 production was produced by cells treated with relatively low doses of EPA or DHA. When high levels of stimulants (1000 mu M EPA or DHA) were used, PGE(2) levels were elevated and reached a significant level (P < 0.05). Both EPA and DHA significantly inhibited the production of sPLA(2), where DHA exerted the more potent inhibitory effects than EPA. No pronounced effect was observed on IL-1 beta production among all the treatments, and IL-1 beta level in cell culture supernatant was fairly low (only approximately 6 pg/ml). Those findings suggested that EPA and DHA could influence the immunity and physiological conditions of macrophages from head kidney of large yellow croaker in vitro. (C) 2013 Published by Elsevier Ltd.
机译：体外研究了不同浓度的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）对分离自大黄鱼的头部肾脏巨噬细胞免疫反应的比较作用。在无血清培养基中放置1天后，将培养的细胞在补充有脂肪酸牛血清形式的EPA或DHA（分别为0、5、25、100、200和1000μM）的分级水平的培养基中孵育白蛋白（FA-BSA）复合物分别持续12 h，24 h和36 h。对照样品在不存在EPA或DHA（2％牛血清白蛋白，BSA）的情况下进行孵育。刺激后，细胞活力，脂质过氧化，秘书磷脂酶A（2）（sPLA（2））和前列腺素E-2（PGE（2））产生以及一些免疫参数，包括吞噬作用，呼吸爆发活性和白介素1β（确定IL-1β）的产生。结果表明，EPA和DHA以剂量依赖性和时间依赖性方式影响细胞活力。特别是，与1000μMEPA或DHA孵育24小时和36小时后，细胞活力显着降低（P <0.05）。较高水平的EPA（200和1000μM）导致丙二醛（MDA）的产量显着增加（P <0.05），而DHA并未显着影响MDA的产量。 EPA显着增加了细胞内超氧阴离子的合成，相反，DHA显着降低了其合成。吞噬作用百分比（PP）值在使用5μMDHA的治疗中显着更高（P <0.05），但是与对照组相比，分别降低了200和1000μMEPA和DHA（P <0.05）。用较低剂量的EPA或DHA处理的细胞产生的PGE2产量减少。当使用高水平的兴奋剂（1000μM EPA或DHA）时，PGE（2）水平升高并达到显着水平（P <0.05）。 EPA和DHA均显着抑制sPLA（2）的产生，其中DHA的抑制作用比EPA更强。在所有处理中均未观察到对IL-1β产生的明显影响，并且细胞培养上清液中的IL-1β水平相当低（仅约6 pg / ml）。这些发现表明，EPA和DHA可能会影响大黄鱼头部肾脏巨噬细胞的免疫力和生理状况。 （C）2013由Elsevier Ltd.出版

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