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首页> 外文期刊>Fish & Shellfish Immunology >A new shrimp peritrophin-like gene from Exopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection
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A new shrimp peritrophin-like gene from Exopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection

机译：克氏Exopalaemon carinicauda的一个新的虾围网素样基因参与白斑综合症病毒（WSSV）感染

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摘要

Peritrophin was first separated from insect peritrophic membrane (PM), and it played an important role in stimulating the digestion of food and protecting insects from invasion by microorganisms. In this study, a full-length cDNA of a new peritrophin-like protein (EcPT) was cloned from the ridgetail white shrimp Exopalaemon carinicauda, which was an excellent experimental animal for shrimp. The full length cDNA comprised 1235 bp including an 873 bp open reading frame encoding 291 amino acids. The deduced amino acid sequence contained a segment of signal peptide and three conserved chitin binding type 2 domains (ChtBD2) characterized by having a 6-cysteine motif. Tissue expression analysis revealed that EcPT was mainly expressed in stomach and gills, which were also the two main target tissues of WSSV infection. The transcription levels of EcPT in both stomach and gills were found to have significantly changed upon WSSV infection by real-time PCR. Silencing EcPT by dsRNA interference led to higher survival rate of shrimp against WSSV challenge, which suggested that EcPT might be involved in WSSV infection. (C) 2013 Elsevier Ltd. All rights reserved.
机译：促营养素首先从昆虫的营养膜上分离出来，在刺激食物的消化和保护昆虫免受微生物入侵方面起着重要的作用。在这项研究中，从山脊白虾Exopalaemon carinicauda克隆了一种新的长养蛋白样蛋白（EcPT）的全长cDNA，该虾是对虾极好的实验动物。全长cDNA包含1235 bp，包括873 bp的开放阅读框，编码291个氨基酸。推导的氨基酸序列包含一段信号肽和三个保守的几丁质结合2型结构域（ChtBD2），其特征是具有6个半胱氨酸基序。组织表达分析表明，EcPT主要在胃和腮中表达，这也是WSSV感染的两个主要目标组织。通过实时PCR检测到WSSV感染后，胃和腮中EcPT的转录水平发生了显着变化。 dsRNA干扰使EcPT沉默导致虾对WSSV攻击的存活率更高，这表明EcPT可能与WSSV感染有关。 （C）2013 Elsevier Ltd.保留所有权利。

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