Cloning and characterization of the trout perforinSofia Athanasopoulou, Dimitra Marioli, Angeliki Mikrou, Anastasios D. Papanastasiou,

Ioannis K. Zarkadis

首页> 外文期刊>Fish & Shellfish Immunology >Cloning and characterization of the trout perforinSofia Athanasopoulou, Dimitra Marioli, Angeliki Mikrou, Anastasios D. Papanastasiou,

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Cloning and characterization of the trout perforinSofia Athanasopoulou, Dimitra Marioli, Angeliki Mikrou, Anastasios D. Papanastasiou,

机译：鳟鱼穿孔素的克隆和鉴定索非亚·阿萨纳索波洛（Sofia Athanasopoulou），迪米特拉·马里奥利（Dimitra Marioli），安吉利基·米库（Angeliki Mikrou），阿纳斯塔索斯·斯·帕帕纳斯塔索

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The pore-forming protein, perforin is one of the effectors of cell-mediated killing. A perforin cDNA clone was isolated from rainbow trout (Oncorhynchus mykiss) after screening of a spleen cDNA library. The fulllength cDNA is 2070 bp in size, encodingfor a polypeptide of 589 amino acids. The predicted amino acid sequence of the trout perforin is 64, 58 and 40% identical to those of Japanese flounder, zebrafish and human perforins, respectively. Although its membrane attack complex/perforin (MACPF) domain isconserved, trout perforin shows low homology to human and trout terminal complement components (C6, C7, C8 and C9), ranging from 19 to 26% identity. Expression analysis reveals that the trout perforin gene is expressed in the blood, brain, heart, kidney,intestine and spleen. Phylogenetic analysis of proteins which belong to the MACPF superfamily clusters the trout perforin in the same group with other known perforins.

机译：孔形成蛋白穿孔素是细胞介导的杀伤作用的一种。在筛选脾脏cDNA文库后，从虹鳟（Oncorhynchus mykiss）中分离出穿孔素cDNA克隆。全长cDNA大小为2070 bp，编码589个氨基酸的多肽。鳟鱼穿孔素的预测氨基酸序列分别与日本比目鱼，斑马鱼和人穿孔素的氨基酸序列分别为64、58和40％。尽管其膜攻击复合物/穿孔素（MACPF）结构域是保守的，但鳟鱼穿孔素与人和鳟鱼末端补体成分（C6，C7，C8和C9）的同源性较低，同一性范围为19％至26％。表达分析表明鳟鱼穿孔素基因在血液，脑，心脏，肾脏，肠和脾中表达。属于MACPF超家族的蛋白质的系统发育分析将鳟鱼穿孔素与其他已知的穿孔素聚集在同一组中。

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《Fish & Shellfish Immunology》 |2009年第6期|共5页
作者
Ioannis K. Zarkadis;
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正文语种 eng
中图分类水产、渔业;
关键词
Pore-forming toxin; MACPF superfamily; Perforin; Evolution; Rainbow trout; Expression; Phylogenetic analysis;

机译：成孔毒素;MACPF超家族;穿孔素;进化;虹鳟鱼;表达;系统发育分析;

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外文文献

1. Cloning and characterization of the trout perforinSofia Athanasopoulou, Dimitra Marioli, Angeliki Mikrou, Anastasios D. Papanastasiou, [J] . Ioannis K. Zarkadis Fish & Shellfish Immunology . 2009,第6期

机译：鳟鱼穿孔素的克隆和鉴定索非亚·阿萨纳索波洛（Sofia Athanasopoulou），迪米特拉·马里奥利（Dimitra Marioli），安吉利基·米库（Angeliki Mikrou），阿纳斯塔索斯·斯·帕帕纳斯塔索
2. Cloning, characterization and genomic structure of the natural killer cell enhancement factor (NKEF)-like gene from homozygous clones of rainbow trout (Oncorhynchus mykiss) [J] . Zhang H., Thorgaard GH., Ristow SS., Developmental and Comparative Immunology: Ontogeny, Phylogeny, Aging: The Official Journal of the International Society of Developmental and Comparative Immunology . 2001,第1期

机译：虹鳟（Oncorhynchus mykiss）纯合子自然杀伤细胞增强因子（NKEF）样基因的克隆，鉴定和基因组结构
3. Cloning, overexpression, and characterization of a bacterial Ca(2+)-dependent phospholipase D. [J] . Yang H, Roberts MF Protein Science: A Publication of the Protein Society . 2002,第12期

机译：克隆，过表达和细菌Ca（2+）依赖磷脂酶D的表征。
4. Cloning and characterization of trout cardiac troponin I. [D] . Kirkpatrick, Kelly Pauline. 2010

机译：鳟鱼心肌肌钙蛋白I的克隆与鉴定
5. Cloning of the rainbow trout (Oncorhynchus mykiss) Mx2 and Mx3 cDNAs and characterization of trout Mx protein expression in salmon cells. [O] . G D Trobridge, P P Chiou, J A Leong 1997

机译：虹鳟Mx2和Mx3 cDNA的克隆以及鲑鱼细胞中鳟Mx蛋白表达的表征。
6. Cloning and characterization of a novel oocyte-specific gene encoding an F-Box protein in rainbow trout (Oncorhynchus mykiss) [O] . Lei Wang, Swamy K Tripurani, Warapond Wanna, 2013

机译：虹鳟鱼（Oncorhynchus mykiss）中编码F-Box蛋白的新型卵母细胞特异性基因的克隆和鉴定

Cloning and characterization of the trout perforinSofia Athanasopoulou, Dimitra Marioli, Angeliki Mikrou, Anastasios D. Papanastasiou,

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