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首页> 外文期刊>Fish & Shellfish Immunology >Amylase and aspartate aminotransferase in the haemolymph of the European flat oyster Ostrea edulis
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Amylase and aspartate aminotransferase in the haemolymph of the European flat oyster Ostrea edulis

机译：欧洲扁平牡蛎食用牡蛎血淋巴中的淀粉酶和天冬氨酸转氨酶

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Comparatively little information is available on the non-cellular haemolymph constituents of the European oyster Ostrea edulis apart from such parameters as protein and lysozyme [1]. During a study of oysters in Cork Harbour on the south coast of Ireland levels of amylase and aspartate aminotransferase were determined in the haemolymph plasma. The levels of these enzymes were subsequently analysed in monthly samples to establish baseline information. The oysters used in this study were 22 months old at the beginning of the study. Approximately 58 oysters were sampled on a monthly basis by dredging a designated bed. It was subsequently observed that oysters from this group initially spawned in the weeks following the first sample (pers. obs.). Seawater temperature (deg C) was measured at a depth of 2 m, the approximate depth of the oyster beds. Amylase levels were measured monthly from June 1990 to May 1991 and aspartate aminotransferase from June 1990 to March 1991. Prevalence of infection of Bonamia ostreae was also screened in the oysters. However, throughout the study period prevalence of infection was low and no significant difference in amylase or aspartate aminotransferase was found between infected and uninfected oysters so all measurements for each month were treated as one group. Haemolymph was removed from the adductor muscle sinus of each oyster and placed in 1.5 ml Eppendorf tubes on ice. The samples were spun on a bench top centrifuge to separate the cells and the supernatant. The plasma component was then removed and placed in clean Eppendorf tubes and held at - 20 deg C until analysis was carried out (generally within 2-4 weeks). To measure both components, plasma was diluted 1:3 with distilled de-ionised water and analysis was carried out automatically in a Hitachi 704 discrete analyser. The number of oysters for which both parameters were measured varied, due to the small quantities of plasma available from individual oysters. Two-way ANOVA following a Log (x+l) transformation was employed to determine the effect of sampling date on the two parameters. These were followed by multiple comparison (Tukey) tests in cases where the ANOVA had shown significant results [2].
机译：除蛋白质和溶菌酶等参数外，关于欧洲牡蛎可食牡蛎的非细胞性血淋巴成分的信息相对较少[1]。在爱尔兰南海岸的科克港的牡蛎研究期间，确定了血淋巴血浆中的淀粉酶和天冬氨酸转氨酶水平。随后在每月的样品中分析这些酶的水平以建立基线信息。本研究中使用的牡蛎在研究开始时为22个月大。通过疏通指定的床，每月大约采样58只牡蛎。随后观察到，该组牡蛎最初是在第一个样本后数周产卵的。在2 m的深度（牡蛎床的近似深度）处测量海水温度（摄氏度）。从1990年6月至1991年5月每月测量淀粉酶水平，从1990年6月至1991年3月每月测量天冬氨酸转氨酶。还对牡蛎中Bonamia ostreae的感染率进行了筛查。但是，在整个研究期间，感染的发生率很低，在感染的牡蛎和未感染的牡蛎之间，淀粉酶或天冬氨酸转氨酶没有显着差异，因此，每个月的所有测量值均被视为一组。从每个牡蛎的内收肌窦中取出血淋巴，然后放入冰中的1.5 ml Eppendorf管中。将样品在台式离心机上旋转以分离细胞和上清液。然后将血浆成分移出并放置在干净的Eppendorf管中，并保持在-20℃，直到进行分析（通常在2-4周内）。为了测量两种成分，将血浆用蒸馏去离子水按1：3稀释，并在Hitachi 704离散分析仪中自动进行分析。由于单个牡蛎的血浆量少，因此测量了这两个参数的牡蛎数量各不相同。采用对数（x + 1）转换后的双向ANOVA来确定采样日期对这两个参数的影响。在方差分析显示出明显结果的情况下，进行多重比较（Tukey）测试[2]。

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