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首页> 外文期刊>Fish & Shellfish Immunology >Antibody responses by chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (Oncorhynchus mykiss) to various protein antigens
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Antibody responses by chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (Oncorhynchus mykiss) to various protein antigens

机译：奇努克鲑鱼（Oncorhynchus tshawytscha）和虹鳟鱼（Oncorhynchus mykiss）对多种蛋白质抗原的抗体反应

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One of the hallmarks of the fish immune response to a foreign protein is the production of antibodies which bind specifically to epitopes of the target molecule. Because that antibody response is the product of interactions between several components of the immune system, quantification of the antibody response to a defined protein provides an attractive approach for examining changes in the humoral arm of the fish disease defences. Measures of the antibody response have been used to help describe the impact of environmental conditions, physiological changes, vaccination, and pathogenic micro-organisms on fish health. Except for vaccine trials and research studies using particular fish pathogens, antigens used in fish studies are often chosen on the basis of their immunogenicity in mammals. The fish antibody response, however, is generally weak compared to the IgG response of mammals. Few laboratory studies have focused on determining which antigens will elicit a strong antibody response in fish. Hodgins et al. examined the immune response of rainbow trout (Oncorhynchus mykiss) to several protein antigens, but the investigators were limited to measuring the specific antibody levels by immunodiffusion and the haemagglutination test. In the current study, a variety of protein antigens were administered to fish under standardised conditions and the resulting antibody responses were compared on the basis of an enzyme-linked immunosorbent assay (ELISA). The goal of this study was to identify one or more immunogenic protein antigens that would be suitable for characterising the effects of various environmental conditions on the humoral response of salmonids. Whenever possible, inexpensive, commercially prepared protein antigens were selected for testing to ensure that each would be readily available to most fish health research laboratories, and of uniform composition.
机译：鱼对外源蛋白质的免疫反应的标志之一是特异性结合靶分子表位的抗体的产生。由于该抗体应答是免疫系统几个组成部分之间相互作用的产物，因此对确定蛋白的抗体应答进行定量分析可为检查鱼类疾病防御体液臂的变化提供一种有吸引力的方法。抗体应答的测量已用于帮助描述环境条件，生理变化，疫苗接种和病原微生物对鱼类健康的影响。除了使用特定鱼类病原体进行的疫苗试验和研究以外，鱼类研究中使用的抗原通常根据其在哺乳动物中的免疫原性来选择。然而，与哺乳动物的IgG反应相比，鱼抗体反应通常较弱。很少有实验室研究集中在确定哪种抗原会引起鱼类强烈的抗体反应。 Hodgins等。他研究了虹鳟（Oncorhynchus mykiss）对几种蛋白质抗原的免疫反应，但研究人员仅限于通过免疫扩散和血凝试验测量特异性抗体水平。在当前的研究中，在标准化条件下将多种蛋白质抗原施用于鱼类，并在酶联免疫吸附测定（ELISA）的基础上比较了产生的抗体反应。这项研究的目的是确定一种或多种免疫原性蛋白抗原，这些抗原适合表征各种环境条件对鲑鱼体液反应的影响。只要有可能，就应选择便宜的商业制备的蛋白质抗原进行测试，以确保每种鱼抗原都容易为大多数鱼类健康研究实验室所用，并且具有统一的组成。

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