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Characterization of four Mx isoforms in the European eel, Anguilla anguilla

机译：欧洲鳗，中的四种Mx异构体的表征

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摘要

Mx protein is known to play an important role in vertebrate immune response to viral infection. In this study, cDNA sequences of four Mx isoforms, designated as MxA, B, C and D were characterized in the European eel, Anguilla anguilla. These sequences contained an open reading frame of 1899, 1896, 1866, 1779 bp, flanked by 95, 53, 138, 69 bp of 5' untranslated region and 389, 241, 136, 124 bp of 3' untranslated region, respectively. A phylogenetic tree constructed with Mx peptide sequences from vertebrates revealed that MxA, C and D in the European eel formed into a clade containing zebrafish MxA and MxB and Mx proteins in other teleosts, whereas MxB in the eel was clustered together with zebrafish MxD, MxG and MxF. The transcription level of all Mx isoforms increased in a poly I:C dose-dependent manner in peripheral blood leukocytes of eels, as revealed by real-time PCR. A further experiment was conducted to reveal the temporal change in expression of these isoforms in various organs/tissues following poly I:C stimulation, and significant increase in expression was observed at various degrees in different organs or in different sampling occasions within the 12 h experimental period. In particular, MxA had the highest level of increase, while MxB had the lowest; and three isoforms, MxA, MxB and MxD had the highest increase in intestine, while the highest increase of MxC expression was observed in liver. These four isoforms of eel Mx are thus expressed differentially, and further work is certainly required to clarify the activity of promoter elements and antiviral activity of these Mx isoforms. (C) 2013 Elsevier Ltd. All rights reserved.
机译：已知Mx蛋白在脊椎动物对病毒感染的免疫反应中起重要作用。在这项研究中，在欧洲鳗，（Anguilla anguilla）中鉴定了四种分别称为MxA，B，C和D的Mx亚型的cDNA序列。这些序列包含1899、1896、1866、1779bp的开放阅读框，其侧翼分别是5'非翻译区的95、53、138、138、69bp和3'非翻译区的389、241、136、124bp。用脊椎动物的Mx肽序列构建的系统树显示，欧洲鳗鱼中的MxA，C和D形成了包含斑马鱼MxA和MxB以及其他硬骨鱼中Mx蛋白的进化枝，而鳗鱼中的MxB与斑马鱼MxD，MxG聚集在一起和MxF。实时PCR显示，鳗鱼外周血白细胞中所有Mx同工型的转录水平以poly I：C剂量依赖性方式增加。进行了进一步的实验以揭示多聚I：C刺激后这些异型在各种器官/组织中的表达随时间的变化，并且在实验的12小时内，在不同器官或不同采样场合中观察到了不同程度的表达显着增加。期。特别是，MxA的增长水平最高，而MxB的增长水平最低; MxA，MxB和MxD这三种同工型在肠道中的增幅最高，而在肝脏中MxC表达的增幅最高。因此，鳗MMx的这四个同工型表达不同，并且肯定需要进一步的工作来阐明启动子元件的活性和这些Mx同工型的抗病毒活性。 （C）2013 Elsevier Ltd.保留所有权利。

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