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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Mediatophore, a protein supporting quantal acetylcholine release.
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Mediatophore, a protein supporting quantal acetylcholine release.

机译：Mediatophore，一种支持定量乙酰胆碱释放的蛋白质。

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摘要

After having reconstituted in artificial membranes the calcium-dependent acetylcholine release step, and shown that essential properties of the mechanism were preserved, we purified from Torpedo electric organ nerve terminals a protein, the mediatophore, able to release acetylcholine upon calcium action. A plasmid encoding for Torpedo mediatophore was introduced into cells deficient for acetylcholine release and for the expression of the cholinergic genomic locus defined by the co-regulated choline acetyltransferase and vesicular transporter genes. The transfected cells became able to release acetylcholine in response to a calcium influx in the form of quanta. The cells had to be loaded with acetylcholine since they did not synthesize it, and without transporter they could not concentrate it in vesicles. We may then attribute the observed quanta to mediatophores. We know from previous works that like the release mechanism, mediatophore is activated at high calcium concentrations and desensitized at low calcium concentrations. Therefore only the mediatophores localized within the calcium microdomain would be activated synchronously. Synaptic vesicles have been shown to take up calcium and those of the active zone are well situated to control the diffusion of the calcium microdomain and consequently the synchronization of mediatophores. If this was the case, synchronization of mediatophores would depend on vesicular docking and on proteins ensuring this process.
机译：在人工膜上重建了钙依赖性乙酰胆碱的释放步骤，并表明该机制的基本特性得以保留后，我们从鱼雷的电器官神经末梢纯化了一种蛋白质，即介电基团，能够在钙作用下释放乙酰胆碱。将编码鱼雷中间膜的质粒引入缺乏乙酰胆碱释放和表达胆碱能基因组基因座的细胞，该胆碱能基因组基因座由共同调节的胆碱乙酰转移酶和囊泡转运蛋白基因定义。转染的细胞能够响应量子形式的钙流入而释放乙酰胆碱。细胞不能被乙酰胆碱合成，因为它们不能合成乙酰胆碱，没有转运蛋白，它们就不能将其浓缩在囊泡中。然后，我们可以将观察到的量子归因于中间隐喻。从以前的工作中我们知道，与释放机制一样，中线团在高钙浓度下被激活而在低钙浓度下被脱敏。因此，只有钙微结构域内的介电体才会被同步激活。突触小泡吸收钙，而活性区的泡位于适当位置以控制钙微结构域的扩散，从而控制中隐足突的同步。如果是这种情况，中隐足同步将取决于囊泡对接和确保这一过程的蛋白质。

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