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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Increased flow rate and papaverine increase K+ exchange in perfused rat hind-limb skeletal muscle.
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Increased flow rate and papaverine increase K+ exchange in perfused rat hind-limb skeletal muscle.

机译：流速增加和罂粟碱增加灌注大鼠后肢骨骼肌中的K +交换。

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摘要

This study tested the hypothesis that increases in perfusate flow rate result in increased rates of unidirectional and net K+ transport in rat hind-limb skeletal muscle at rest. Ten neurally and vascularly isolated hind limbs, with arterial and venous catheters placed proximal to the popliteal region, were perfused for 10-min periods at flow rates (presented in a random order) of 0.27, 0.42, 0.63, 0.84, or 1.05 mL x min(-1) x g(-1). Potassium extraction and unidirectional K+ influx were determined using 42K, and arterial perfusion pressure was measured continuously. Increases in flow rate resulted in decreases in K+ extraction and increases in unidirectional K+ influx, unidirectional K+ efflux, and net K+ efflux. The increases in K+ flux were associated with increases in oxygen uptake, glucose uptake, and lactate release. In separate experiments (n = 5), the vasodilator papaverine (10(-4) M) did not further vasodilate the vasculature of resting hind limbs, suggesting that the hind limbs in this preparation were fully vasodilated. Papaverine, at constant flow, resulted in a nearly 1.5-fold increase in K+ extraction, a doubling of unidirectional K+ influx, and increases in unidirectional K+ efflux and net K+ efflux. It is concluded that physiological increases in flow rate result in increases in K+ transport in isolated, perfused rat hind-limb skeletal muscle. Furthermore, papaverine appeared to induce an increase in skeletal muscle membrane permeability to K+.
机译：这项研究检验了假说，即灌流液流量增加导致大鼠后肢静止骨骼肌单向和净K +传输速率增加。十根神经和血管隔离的后肢，将动脉和静脉导管放置在pop区的近侧，以0.27、0.42、0.63、0.84或1.05 mL x的流速（以随机顺序显示）灌注10分钟。 min（-1）xg（-1）。使用42K测定钾提取量和单向K +流入量，并连续测量动脉灌注压力。流速增加导致K +提取量减少，单向K +流入量，单向K +流出量和净K +流出量增加。 K +通量的增加与氧吸收，葡萄糖吸收和乳酸释放的增加有关。在单独的实验（n = 5）中，血管扩张药罂粟碱（10（-4）M）并未进一步使静息后肢的血管扩张，这表明该制剂中的后肢已完全扩张。罂粟碱在恒定流量下导致K +提取增加近1.5倍，单向K +流入增加一倍，单向K +流出和净K +流出增加。结论是，生理性流速增加导致离体，灌注大鼠后肢骨骼肌中钾离子转运增加。此外，罂粟碱似乎诱导骨骼肌膜对K +的通透性增加。

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