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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria
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Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria

机译：同时传导图谱和孤立心房中的细胞内膜电位记录

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摘要

We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 x 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 M Omega) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.
机译：我们描述了一种新颖的方法，可以同时确定孤立的心房中动作电位（AP）形态和组织电生理特性的区域差异。将大鼠心房制剂的心外膜表面与多电极阵列（9 x 10个氯化银电极，直径为0.1 mm，间距为0.1 mm）接触。将玻璃微电极（100 M Omega）同时插入心内膜表面，以在从组织的两个相对角起搏时记录来自两个区域（A，B）之一的细胞内AP。当AP在不同的刺激部位启动时，在重新极化80％时的AP持续时间及其恢复曲线仅在A区显着不同（p <0.01）。在2个单独的心律失常发作期间观察到AP持续时间，AP振幅以及传导速度的交替变化。这种将微电极阵列和细胞内膜电位记录相结合的方法可能为心血管疾病动物模型中的心律失常机制提供新的见解。

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