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首页> 外文期刊>Fish & Shellfish Immunology >A Toll receptor from Chinese shrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection.
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A Toll receptor from Chinese shrimp Fenneropenaeus chinensis is responsive to Vibrio anguillarum infection.

机译：中国虾粉对虾的收费受体对鳗弧菌感染有反应。

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摘要

Toll-like receptors (TLRs) are an evolutionarily ancient family of pattern recognition receptors (PRRs), playing a crucial role in innate immune responses. Here we present a Toll homolog from Chinese shrimp Fenneropenaeus chinensis, designated FcToll. The full-length cDNA of FcToll is 4115bp including a poly A-tail of 16bp, encoding a putative protein of 931 amino acids. The predicted protein consists of an extracellular domain with a potential signal peptide, 16 leucine-rich repeats (LRR), two LRR-C-terminal (LRR-CT) motifs, and two LRR-N-terminal (LRR-NT) motifs, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic Toll/Interleukin-1R (TIR) domain of 139 residues. Genomic structure of FcToll gene contains five exons and four introns. Phylogenetic analysis revealed that it belongs to insect-type invertebrate Toll family. Transcripts of FcToll gene were constitutively expressed in various tissues, with predominant level in lymphoid organ. Real-time PCR assays demonstrated that expression patterns of FcToll were distinctly modulated after bacterial or viral stimulation, with significant enhancement after 5h post-Vibrio anguillarum challenge but markedly reduced levels immediately after white spot syndrome virus (WSSV) exposure. These results suggest that FcToll might be involved in innate host defense, especially against the pathogen V. anguillarum.
机译：Toll样受体（TLR）是进化上很古老的模式识别受体（PRR）家族，在先天免疫反应中起着至关重要的作用。在这里，我们介绍了来自中国虾虾（Fenneropenaeus chinensis）的Toll同源物，命名为FcToll。 FcToll的全长cDNA为4115bp，包括16bp的多聚A尾，编码931个氨基酸的推测蛋白。预测的蛋白质由具有潜在信号肽的细胞外结构域，16个富含亮氨酸的重复（LRR），两个LRR-C末端（LRR-CT）基序和两个LRR-N末端（LRR-NT）基序组成，其次是23个氨基酸的跨膜区段，以及139个残基的胞质Toll / Interleukin-1R（TIR）结构域。 FcToll基因的基因组结构包含五个外显子和四个内含子。系统发育分析表明它属于昆虫型无脊椎动物Toll家族。 FcToll基因的转录本在各种组织中组成性表达，在淋巴器官中占主导水平。实时PCR分析表明，在细菌或病毒刺激后，FcToll的表达模式受到明显调节，在鳗弧菌攻击后5h显着增强，但在暴露白斑综合症病毒（WSSV）后立即显着降低水平。这些结果表明，FcToll可能参与了先天宿主防御，特别是针对病原体V. anguillarum的防御。

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