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首页> 外文期刊>Fish & Shellfish Immunology >Cloning and molecular characterization of complement component 1 inhibitor (C1INH) and complement component 8 beta (C8 beta) in Nile tilapia (Oreochromis niloticus)
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Cloning and molecular characterization of complement component 1 inhibitor (C1INH) and complement component 8 beta (C8 beta) in Nile tilapia (Oreochromis niloticus)

机译：尼罗罗非鱼（Oreochromis niloticus）中补体成分1抑制剂（C1INH）和补体成分8 beta（C8 beta）的克隆和分子表征

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Nile tilapia (Oreochromis niloticus), one of the most important groups of food fishes in the world, has frequently suffered from serious challenge from pathogens in recent years. Immune responses of Nile tilapia should be understood to protect the aquaculture industry of this fish. The complement system has an important function in recognizing bacteria, opsonizing these pathogens by phagocytes, or killing them by direct lysis. In this study, two Nile tilapia complement component genes, complement component 1 inhibitor (C1INH) and complement component 8 beta subunit (C8 beta), were cloned and their expression characteristics were analyzed. C1INH cDNA was found containing a 1791 bp open reading frame (ORF) encoding a putative protein with 597 amino acids, a 101 bp 5'-untranslated region (UTR) and a 236 bp 3'-UTR. The predicted protein structure for this gene consisted of two Ig-like domains and glycosyl hydrolase family-9 active site signature 2. The C8 beta cDNA consisted of a 1761 bp ORF encoding 587 amino acids, a 15 bp 5'-UTR and a 170 bp 3'-UTR. The predicted protein of C8 beta contained three motifs, thrombospondin type-1 repeat, membrane attack complex/perforin domain, and LDL-receptor class A. Expression analysis revealed that these two complement genes were highly expressed in the liver, however, were weakly expressed in the gill, heart, brain, kidney, intestine, spleen and dorsal muscle tissues. The present study provided insights into the complement system and immune functions of Nile tilapia. (C) 2013 Elsevier Ltd. All rights reserved.
机译：尼罗罗非鱼（Oreochromis niloticus）是世界上最重要的食用鱼类之一，近年来，它经常遭受病原体的严重挑战。应理解尼罗罗非鱼的免疫反应，以保护该鱼的水产养殖业。补体系统在识别细菌，通过吞噬细胞调理这些病原体或通过直接裂解杀死它们方面具有重要功能。在这项研究中，克隆了两个尼罗罗非鱼补体成分基因，补体成分1抑制剂（C1INH）和补体成分8 beta亚基（C8 beta），并分析了它们的表达特性。发现C1INH cDNA包含一个1791 bp的开放阅读框（ORF），其编码带有597个氨基酸的推定蛋白质，101 bp的5'-非翻译区（UTR）和236 bp的3'-UTR。该基因的预测蛋白质结构由两个Ig样结构域和糖基水解酶家族9活性位点签名2组成。C8beta cDNA由1761 bp的ORF组成，编码587个氨基酸，15 bp的5'-UTR和170 bp 3'-UTR。 C8 beta的预测蛋白包含三个基序：血小板反应蛋白1型重复序列，膜攻击复合物/穿孔素结构域和LDL受体A类。表达分析显示这两个补体基因在肝脏中高表达，但表达较弱。在腮，心脏，大脑，肾脏，肠，脾和背肌组织中。本研究提供了对尼罗罗非鱼的补体系统和免疫功能的见解。 （C）2013 Elsevier Ltd.保留所有权利。

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