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首页> 外文期刊>Fish & Shellfish Immunology >Calreticulin is a microbial-binding molecule with phagocytosis-enhancing capacity
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Calreticulin is a microbial-binding molecule with phagocytosis-enhancing capacity

机译：钙网蛋白是一种具有吞噬作用的微生物结合分子

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Calreticulin (CRT) is a highly conserved calcium-binding protein mainly involved in directing proper conformation of proteins and controlling calcium level. Accumulating data also show that CRT is emerging as an immune-relevant molecule. In this study, we demonstrated that the CRT gene from the amphioxus Branchiostoma japonicum, named Bjcrt, consisted of a signal peptide, three domains (N-, P-, C-domains) and an ER retrieval signal sequence (KDEL), which appears to be the ancient form of vertebrate CRTs, and Bjcrt was expressed in a tissue-specific manner, with the most abundant expression in the notochord. We also demonstrated for the first time that the recombinant BjCRT (rBjCRT) was able to bind the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus aureus. Moreover, both BjCRT as well as human recombinant calreticulin were able to promote the phagocytosis of E. coli and S. aureus by sea bass macrophages. These results indicate that CRT is a microbial-binding molecule and possesses an ability to enhance phagocytosis, a novel function assigned to CRT, reenforcing the notion that CRT is an immune-relevant molecule associated with host immune responses. (C) 2013 Elsevier Ltd. All rights reserved.
机译：钙网蛋白（CRT）是一种高度保守的钙结合蛋白，主要参与指导蛋白质的正确构象和控制钙水平。越来越多的数据还表明，CRT正在作为一种免疫相关分子出现。在这项研究中，我们证明了来自两栖类日本分支的CRT基因，名为Bjcrt，由一个信号肽，三个结构域（N-，P-，C-结构域）和一个ER检索信号序列（KDEL）组成。是脊椎动物CRT的古老形式，Bjcrt以组织特异性方式表达，在脊索中表达最丰富。我们还首次证明了重组BjCRT（rBjCRT）能够结合革兰氏阴性细菌大肠杆菌和革兰氏阳性细菌金黄色葡萄球菌。此外，BjCRT以及人类重组钙网蛋白都能够促进鲈鱼巨噬细胞吞噬大肠杆菌和金黄色葡萄球菌。这些结果表明，CRT是一种微生物结合分子，具有增强吞噬作用的能力，吞噬作用是CRT的一项新功能，强化了CRT是与宿主免疫反应相关的免疫相关分子的观念。 （C）2013 Elsevier Ltd.保留所有权利。

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