...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish & Shellfish Immunology >Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in turbot (Scophthalmus maximus).
【24h】

Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in turbot (Scophthalmus maximus).

机译：大菱turbo中的铁代谢功能和铁调素及其相关基因（铁转运蛋白1和转铁蛋白受体）的潜在抗菌作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Antimicrobial peptide plays an important role in fish immunity. The small molecular antimicrobial peptide Hepcidin in turbot was studied and reported in this paper. The Ferroportin 1 (FPN1) and Transferrin Receptor (TFR) genes, which are related to Hepcidin, were cloned in turbot. The characteristics of Hepcidin and its related genes were studied, including an analysis of the expression patterns and cloning of the Hepcidin promoter, the relationship between Hepcidin and NF- kappa B and the regulation of iron-metabolism. The results showed that the promoter of SmHepcidin contains the binding sites of NF- kappa B, and NF- kappa B may directly or indirectly receive feedback signals from SmHepcidin. In the liver, spleen and kidney, in which there was an increased SmHepcidin expression level, SmFPN1 dramatically decreased and SmTFR was also either decreased or exhibited no obvious change after bacterial/viral infection and an injection of exogenous Hepcidin protein. RNAi experiments in turbot kidney cells confirmed the expression changes of these gene patterns. Furthermore, the administration of exogenous Hepcidin protein, which regulates the level of chelatable iron in cells, further confirmed the function of Hepcidin in iron metabolism. It is speculated that the rapidly increased expression of SmHepcidin may induce changes in the expression of related genes, and that the in vivo chelatable iron concentration which participates in the antibacterial process was also changed when exogenous pathogens are present in turbot. It is suggested that SmHepcidin plays a defensive role against pathogenic infection.
机译：抗菌肽在鱼类免疫中起重要作用。研究并报道了大菱molecular中的小分子抗菌肽海普丁。将与铁调素相关的Ferroportin 1（FPN1）和转铁蛋白受体（TFR）基因克隆到大菱t中。研究了铁调素及其相关基因的特性，包括分析铁调素启动子的表达方式和克隆，铁调素与NF-κB的关系以及铁代谢的调控。结果表明，SmHepcidin的启动子包含NF-κB的结合位点，NF-κB可能直接或间接接收来自SmHepcidin的反馈信号。在肝脏，脾脏和肾脏中，SmHepcidin表达水平升高，SmFPN1显着降低，SmTFR也降低，或者在细菌/病毒感染和注射外源Hepcidin蛋白后无明显变化。菱形肾细胞中的RNAi实验证实了这些基因模式的表达变化。此外，调节细胞中可螯合的铁水平的外源性铁调素蛋白的施用进一步证实了铁调素在铁代谢中的功能。据推测，SmHepcidin的迅速增加的表达可能诱导相关基因表达的变化，并且当大菱t中存在外源病原体时，参与抗菌过程的体内可螯合铁浓度也发生了变化。提示SmHepcidin对病原体感染起防御作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号