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首页> 外文期刊>Fish & Shellfish Immunology >Combined effects of high stocking density and Piscirickettsia salmonis treatment on the immune system, metabolism and osmoregulatory responses of the Sub-Antarctic Notothenioid fish Eleginops maclovinus
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Combined effects of high stocking density and Piscirickettsia salmonis treatment on the immune system, metabolism and osmoregulatory responses of the Sub-Antarctic Notothenioid fish Eleginops maclovinus

机译：高放养密度和鲑鱼立克次氏体处理对南极亚种类异戊二烯类鱼类Eleginops maclovinus免疫系统，代谢和渗透调节反应的综合影响

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摘要

The aim of this study was to evaluate immunological, metabolic and osmoregulatory secondary stress responses in Eleginops maclovinus specimens submitted to three different stocking densities: i) low (3.1 kg m(-3)), medium (15 kg m(-3)) and high (60 kg m(-3)) during 10 days, alone or in combination with a previous treatment of a protein extract of the pathogen Piscirickettsia salmonis (0.5 mu g g weight body(-1)). Plasma, liver, gill and kidney samples were obtained at the end of both experiments. Plasma cortisol and amino acid levels increased, while plasma glucose, triglyceride and lactate levels decreased at higher stocking densities. However, no effects were observed on serum Immunoglobulin type M (IgM anti P. salmonis level) values. Gill Na+, K+-ATPase activity enhanced under these experimental conditions, suggesting an osmotic imbalance. Energy metabolism changes, assessed by metabolite concentrations and enzyme activities, indicated a reallocation of energetic substrates at higher stocking densities. Specimens inoculated with a protein extract of P. salmonis and maintained at different stocking densities showed primary stress response, as all groups enhanced plasma cortisol concentrations. Serum IgM levels increased after treatment with P. salmonis extract but a negative influence of high stocking density on IgM production was observed when immune system was activated. Furthermore, treatment with P. salmonis protein extract evoked deep changes in the metabolite stores in all tissues tested, indicating a mobilization of energy substrates in response to infection. The results show that stocking density induced immunological, metabolic and osmoregulatory secondary stress responses in E. maclovinus specimens and that previous treatment with P. salmonis compromise these changes
机译：这项研究的目的是评估提交到三种不同放养密度下的Eleginops maclovinus标本的免疫，代谢和渗透调节性继发应激反应：i）低（3.1 kg m（-3）），中（15 kg m（-3））且在10天内达到最高（60 kg m（-3）），单独或与先前处理过的病原体鲑鱼立克次氏体（0.5 mu gg体重（-1））的蛋白质提取物组合使用。在两个实验结束时获得血浆，肝，腮和肾样品。在较高的放养密度下，血浆皮质醇和氨基酸水平增加，而血浆葡萄糖，甘油三酸酯和乳酸水平下降。但是，没有观察到对血清M型免疫球蛋白（IgM抗鲑鱼假单胞菌水平）值的影响。在这些实验条件下，ill Na +，K + -ATPase活性增强，表明渗透失衡。通过代谢物浓度和酶活性评估的能量代谢变化表明，高放养密度下高能底物的重新分配。接种鲑鱼蛋白提取物并保持在不同放养密度下的标本显示出主要的应激反应，因为所有组均提高了血浆皮质醇浓度。用鲑鱼假单胞菌提取物处理后，血清IgM水平升高，但是当免疫系统被激活时，观察到高饲养密度对IgM产生负面影响。此外，用鲑鱼毕赤酵母蛋白提取物进行的治疗引起了所有测试组织中代谢物储藏的深刻变化，表明能量底物可响应感染而动员。结果表明，放养密度可引起Maclovinus样本中的免疫，代谢和渗透调节性继发应激反应，而以前用鲑鱼假单胞菌处理会损害这些变化。

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