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首页> 外文期刊>Fish & Shellfish Immunology >The coelomic fluid of the sea urchin Tripneustes depressus shows antiviral activity against Suid herpesvirus type 1 (SHV-1) and rabies virus (RV).
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The coelomic fluid of the sea urchin Tripneustes depressus shows antiviral activity against Suid herpesvirus type 1 (SHV-1) and rabies virus (RV).

机译：海胆中曲霉的腔体液对1型Suid疱疹病毒（SHV-1）和狂犬病病毒（RV）具有抗病毒活性。

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Several studies have reported that molecules extracted from invertebrates have activity against different viruses, even against those that do not infect these organisms in their environment. One of the main mechanisms against pathogens in these organisms is the production of antimicrobial peptides. The objective of this study was to determine whether the coelomic fluid (CF) of the sea urchin Tripneustes depressus has activity against Suid herpesvirus type 1 (SHV-1) and/or rabies virus (RV). We tested the antiviral activity of CF in neutralizing assays and observed 50% inhibition against SHV-1 lytic plaque formation using 33 micro g of CF, whereas 21 micro g CF was sufficient to obtain more than 90% inhibition for RV. Cytotoxicity to MDBK and BHK-21 cells was found with whole CF yet was eliminated by heating at 56 or 72 degrees C (even when using 50 micro g of heat-inactivated CF supernatant [SN or thermostable fraction]), and SN retained the antiviral effect. In both cases, the antiviral effect was direct and thermostable (SN 56 and 72 degrees C), and the best inhibition was observed when CF+virus was incubated prior to the addition of the cells. Therefore, the coelomic fluid of T. depressus has antiviral activity against SHV-1 and RV that is direct and stable at 72 degrees C. We suggest that further assays should be performed using more accurate methods to characterize new molecules with antiviral activity that may result in new drugs.
机译：几项研究报告说，从无脊椎动物中提取的分子具有针对不同病毒的活性，甚至针对在其环境中未感染这些生物的病毒。在这些生物中抵抗病原体的主要机制之一是产生抗菌肽。这项研究的目的是确定海胆中曲霉的腔体液（CF）是否具有抗Suid 1型疱疹病毒（SHV-1）和/或狂犬病病毒（RV）的活性。我们在中和试验中测试了CF的抗病毒活性，并使用33微克CF观察到对SHV-1溶菌斑形成有50％的抑制作用，而21微克CF足以获得超过90％的RV抑制作用。发现完整的CF对MDBK和BHK-21细胞具有细胞毒性，但在56或72°C加热时已消除（即使使用50微克热灭活的CF上清液[SN或热稳定级分]），并且SN保留了抗病毒药影响。在这两种情况下，抗病毒作用都是直接的和热稳定的（SN 56和72摄氏度），并且在添加细胞之前孵育CF +病毒时观察到最佳的抑制作用。因此，T。depressus的结肠液对SHV-1和RV具有直接的抗病毒活性，并在72摄氏度下稳定。我们建议应使用更准确的方法进行进一步的测定，以鉴定具有抗病毒活性的新分子。在新药中。

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