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首页> 外文期刊>Fish & Shellfish Immunology >Humoral immune response and TLR9 gene expression in Pacific red snapper (Lutjanus peru) experimentally exposed to Aeromonas veronii
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Humoral immune response and TLR9 gene expression in Pacific red snapper (Lutjanus peru) experimentally exposed to Aeromonas veronii

机译：实验性暴露于维罗纳气单胞菌的太平洋红鲷（Lutjanus peru）的体液免疫应答和TLR9基因表达

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摘要

Aquaculture production of Pacific red snapper Lutjanus peru is growing rapidly in Mexico, especially in Gulf of California. As it is a relatively new aquaculture species there are few reports evaluating its immune response to pathogens. The Gram-negative bacteria Aeromonas veronii is a heterogeneous organism that causes the disease known as motile aeromonad septicemia, which is responsible for serious economic loss in seabream culture due to bacterial infections. For the purpose of this study, juvenile Pacific red snapper specimens were intraperitoneally injected with low doses of A. veronii (1 x 10(6) CFU ml(-1)). Changes in humoral immune parameters (total protein, myeloperoxidase, lisozyme and antiprotease activities and IgM levels), as well as superoxide dismutase and catalase activities, and TLR9 gene expression were evaluated 24 and 48 h after injection. Overall, the results showed an enhanced in humoral immune parameters and SOD and CAT activities in fish infected with A. veronii compared with control group at 24 or 48 h. By real time PCR assays, the basal mRNA transcripts of TLR9 showed that were highly expressed in intestine and leucocytes compared to skin, head kidney, liver and gill. Then, the mRNA expression levels of TLR9 in head kidney, skin, liver and intestine were analyzed in non-infected and experimentally infected fish 24 and 48 h after injection. A. veronii up-regulated the expression of TLR9 at 24 or 48 h of exposure in all samples analyzed except in liver. Interestingly, intestine produced the greatest increase in transcript levels upon exposure (48 h) to A. veronii. Taken together, our results suggest that low doses of A. veronii infection inducing humoral immune system and TLR9 immune gene in Pacific red snapper that can be useful in the health control of this species. (C) 2014 Elsevier Ltd. All rights reserved.
机译：墨西哥，特别是加利福尼亚湾，太平洋红鲷鱼Lutjanus peru的水产养殖产量增长迅速。由于它是一个相对较新的水产养殖物种，因此鲜有评估其对病原体免疫应答的报道。革兰氏阴性菌维罗尼氏气单胞菌是一种异质性生物，会引起被称为运动性气单胞菌败血症的疾病，该病是由于细菌感染导致鲷鱼养殖严重经济损失的原因。出于本研究的目的，向幼年太平洋红鲷鱼标本腹膜内注射低剂量的维氏曲霉（1 x 10（6）CFU ml（-1））。注射后24小时和48小时，评估体液免疫参数的变化（总蛋白，髓过氧化物酶，同工酶和抗蛋白酶活性以及IgM水平）以及超氧化物歧化酶和过氧化氢酶活性以及TLR9基因表达。总体而言，结果显示，与对照组相比，在24或48小时时，感染了A. veronii的鱼的体液免疫参数以及SOD和CAT活性增强。通过实时PCR分析，TLR9的基础mRNA转录本显示与皮肤，头部肾脏，肝脏和g相比，其在肠和白细胞中的表达较高。然后，在注射后24小时和48小时，分析未感染和实验感染的鱼的头部肾脏，皮肤，肝脏和肠道中TLR9的mRNA表达水平。 Veronii在所有被分析的样品中（肝脏除外）在暴露后24或48 h上调TLR9的表达。有趣的是，肠暴露于ver.ii后（48小时），其转录水平增加最大。综上所述，我们的研究结果表明，低剂量的维氏假单胞菌感染会在太平洋红鲷鱼中诱导体液免疫系统和TLR9免疫基因，可用于对该物种的健康控制。 （C）2014 Elsevier Ltd.保留所有权利。

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