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首页> 外文期刊>Fish & Shellfish Immunology >Frequent occurrence of apoptosis is not associated with pathogenic infectious pancreatic necrosis virus (IPNV) during persistent infection
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Frequent occurrence of apoptosis is not associated with pathogenic infectious pancreatic necrosis virus (IPNV) during persistent infection

机译：持续感染期间细胞凋亡的频繁发生与病原性传染性胰腺坏死病毒（IPNV）不相关

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摘要

Infectious pancreatic necrosis virus (IPNV), a member of the genus Aquabirnavirus and family Birnaviridae, is an unenveloped icosahedral virus with two segments of double-stranded RNA. IPNV causes acute infection in salmornid fry and fingerlings with high mortality. However, this mortality is low as the age increases and survivors become IPNV-carrier fish. In this study, IPNV persistent infection was established in rainbow trout with no clinical signs or mortality. TUNEL staining and immunohistochemistry showed that IPNV antigen-positive cells did not have an apoptotic nucleus in almost all tissue sections and leucocyte smears, indicating that apoptosis was not induced in IPN-V antigen-positive cells. The IPNV genome detected by in situ RT-PCR was more frequent than detection of the IPNV antigen by immumohistochemistry in the kidney, spleen, and liver. This result implies that the successive replication would not occur in many IPNV-infected cells. Further, apoptotic cells were predominant in the tissue sections where the signal-positive cells were frequently detected. Therefore, the presence of apoptosis in this study might be associated with host defense mechanisms, which eliminates IPNV-infected cells by the recognition of IPNV genome at the early stage of infection.
机译：传染性胰腺坏死病毒（IPNV）是Aquabirnavirus属和Birnaviridae家族的成员，是一种非包膜的二十面体病毒，具有两个双链RNA片段。 IPNV引起沙门氏菌鱼苗和鱼种的急性感染，死亡率很高。但是，随着年龄的增长，这种死亡率很低，幸存者成为IPNV携带者。在这项研究中，在虹鳟鱼中建立了IPNV持续感染，没有临床症状或死亡。 TUNEL染色和免疫组化显示，IPNV抗原阳性细胞几乎在所有组织切片和白细胞涂片中均没有凋亡核，这表明IPN-V抗原阳性细胞未诱导凋亡。通过原位RT-PCR检测到的IPNV基因组比通过免疫组织化学在肾脏，脾脏和肝脏中检测到IPNV抗原的频率更高。该结果暗示在许多感染了IPNV的细胞中不会发生连续复制。此外，在经常检测到信号阳性细胞的组织切片中，凋亡细胞占主导。因此，本研究中凋亡的存在可能与宿主防御机制有关，该机制通过在感染初期识别IPNV基因组而消除了感染IPNV的细胞。

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