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首页> 外文期刊>Fish & Shellfish Immunology >The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus.
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The immunostimulatory effect of hot-water extract of Gracilaria tenuistipitata on the white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus.

机译：江Gra提取物对白虾凡纳滨对虾的免疫刺激作用及其对溶藻弧菌的抵抗力。

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摘要

The total haemocyte count (THC), phenoloxidase activity, respiratory burst, superoxide dismutase (SOD) activity, phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were examined in the white shrimp Litopenaeus vannamei (10.3+or-1.5 g) injected individually with hot-water extract of Gracilaria tenuistipitata at 4 or 6 micro g g-1. L. vannamei receiving hot-water extract of G. tenuistipitata at either dose increased significantly its THC, phenoloxidase activity, and respiratory burst after 2 days. L. vannamei received hot-water extract of G. tenuistipitata at 6 micro g g-1 increased its phagocytic activity and clearance efficiency to V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with hot-water extract of G. tenuistipitata were challenged with V. alginolyticus at 2x106 colony-forming units (cfu) shrimp-1 and then placed in seawater of 34 per mil. The survival of shrimp that received hot-water extract of G. tenuistipitata at 6 micro g g-1 was significantly higher than that of shrimp that received saline and the control shrimp after 3 days, and at the termination of the experiment (6 days after the challenge). It is therefore concluded that L. vannamei receiving the hot-water extract of G. tenuistipitata at 6 micro g g-1 or less increased its immune ability and resistance to V. alginolyticus infection.
机译：在分别注射南美白对虾南美白对虾（10.3+或-1.5 g）的白虾对虾中检查总血细胞计数（THC），酚氧化酶活性，呼吸爆发，超氧化物歧化酶（SOD）活性，吞噬活性和对溶藻弧菌的清除效率。 Gracilaria tenuistipitata的热水提取物为4或6 micro g g-1。任一种剂量的南美白对虾的热水提取物在两天后均显着提高其四氢大麻酚，酚氧化酶活性和呼吸爆发。 1天后，南美白对虾以6微克g-1的浓度提取了tenuistipitata的热水提取物，提高了吞噬活性和对溶藻弧菌的清除效率。在另一个实验中，注射了G. tenuistipitata热水提取物的南美白对虾用溶藻弧菌以2x106菌落形成单位（cfu）虾1攻击，然后置于每密耳34的海水中。 3天后以及实验结束后（第6天），接受6克g-1的ten.stipitpitata热水提取物的虾的存活率显着高于接受盐水和对虾的虾。挑战）。因此可以得出结论，凡纳滨对虾在6微克g-1或更少的条件下接受tenuistipitata的热水提取物，可以增强其免疫能力和对溶藻弧菌的抵抗力。

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