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首页> 外文期刊>Fish & Shellfish Immunology >Mutations in the Aeromonas salmonicida subsp. salmonicida type III secretion system affect Atlantic salmon leucocyte activation and downstream immune responses
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Mutations in the Aeromonas salmonicida subsp. salmonicida type III secretion system affect Atlantic salmon leucocyte activation and downstream immune responses

机译：鲑鱼气单胞菌亚种中的突变。鲑鱼III型分泌系统影响大西洋鲑鱼白细胞活化和下游免疫反应

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摘要

Deletion mutants of Aeromonas salmonicida subsp. salmonicida were used to determine the effect of the type three secretion system (TTSS) on Atlantic salmon anterior head kidney leucocytes (AHKL). One strain had a deletion in the outer membrane pore gene, ascC; and the other in three effector genes: aopO, aopH and aexT (we call this strain Delta aop3). Host cell invasion success and 24h survival were depressed in Delta ascC, as was 24h survival of Delta aop3, when compared to the wild type strain. Challenge of AHKLs with A449 or TTSS mutants stimulated expression of the inflammatory mediators IL-8, IL-1 and TNF alpha at two bacterial concentrations (A sub(6) sub(0) sub(0) 0.1, 0.01). Expression of IL-12 was not stimulated in Delta ascC challenged cells, whereas A449 and Delta aop3 challenge resulted in an up-regulation of IL-12 in AHKLs, 2- and 4-fold higher than PBS, respectively. Only the wild type strain elicited a significant increase in IL-10 expression (5.5x at A sub(6) sub(0) sub(0) 0.1). Inducible nitric oxide synthetase (iNOS) and arginase (I+II) genes were also significantly up-regulated upon exposure to all strains. However, iNOS:arginase ratio was elevated in the effector mutant challenge. These results suggest that A. salmonicida subsp. salmonicida may enhance survival within the host cell through polarization of macrophages/leucocytes to an alternative, rather than classical, activation state. Furthermore, the short-term survival and lack of T-cell signalling cytokine stimulation in Delta ascC, may help explain its inefficiency at providing protection to subsequent wild type challenge.
机译：鲑气单胞菌亚种的缺失突变体。鲑鱼被用于确定三型分泌系统（TTSS）对大西洋鲑鱼前脑肾白细胞（AHKL）的作用。一种菌株的外膜孔基因ascC缺失。另一个是三个效应基因：aopO，aopH和aexT（我们将其称为Delta aop3菌株）。与野生型菌株相比，Delta ascC的宿主细胞入侵成功率和24h存活率均受到抑制，Delta aop3的24h存活率也受到抑制。用A449或TTSS突变体对AHKL的攻击刺激了两种细菌浓度下的炎性介质IL-8，IL-1和TNFα的表达（A sub（6）sub（0）sub（0）0.1，0.01）。 IL-12的表达在Delta ascC攻击的细胞中未受到刺激，而A449和Delta aop3攻击导致AHKL中IL-12的上调，分别比PBS高2倍和4倍。仅野生型菌株引起IL-10表达的显着增加（A sub（6）sub（0）sub（0）0.1为5.5x）。暴露于所有菌株后，诱导型一氧化氮合成酶（iNOS）和精氨酸酶（I + II）基因也显着上调。然而，在效应突变体攻击中，iNOS：精氨酸酶的比例增加了。这些结果表明，沙门氏菌亚种。鲑鱼科可以通过将巨噬细胞/白细胞极化为另一种而非经典的活化状态来提高宿主细胞内的存活率。此外，Delta ascC的短期存活和T细胞信号传导细胞因子刺激的缺乏，可能有助于解释其在为随后的野生型攻击提供保护方面的效率低下。

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