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首页> 外文期刊>Fish & Shellfish Immunology >Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata.
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Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata.

机译：珍珠牡蛎Pinctada fucata胞质Cu / Zn-超氧化物歧化酶（SOD）的分子克隆，表征和表达分析。

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摘要

Because of its capacity to rapidly convert superoxide to hydrogen peroxide, superoxide dismutase (SOD) is crucial in both intracellular signalling and regulation of oxidative stress. In this paper we report the cloning of a Cu/Zn SOD (designated as pfSOD) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this Cu/Zn SOD contains an open reading frame (ORF) of 471 bp coding for 156 amino acids. No signal peptide was identified at the N-terminal amino acid sequence of Cu/Zn SOD indicating that this pfSOD encodes a cytoplasmic Cu/Zn SOD. This is supported by the presence of conserved amino acids required for binding copper and zinc. Semi-quantitative analysis in adult tissues showed that the pfSOD mRNA was abundantly expressed in haemocytes and gill and scarcely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfSOD mRNA in haemocytes was increased, reaching the highest level at 8 h, then dropping to basal levels at 36 h. These results suggest that Cu/Zn SOD might be used as a bioindicator of the aquatic environmental pollution and cellular stress in pearl oyster.
机译：由于其能够将超氧化物快速转化为过氧化氢，因此超氧化物歧化酶（SOD）在细胞内信号传导和氧化应激调节中都至关重要。在本文中，我们报告了通过快速扩增cDNA末端（RACE）PCR从珍珠贝（Pinctada fucata）中克隆Cu / Zn SOD（称为pfSOD）的方法。该Cu / Zn SOD的全长cDNA包含一个471 bp的开放阅读框（ORF），编码156个氨基酸。在Cu / Zn SOD的N端氨基酸序列未发现信号肽，表明该pfSOD编码胞质Cu / Zn SOD。这由结合铜和锌所需的保守氨基酸的存在来支持。在成人组织中的半定量分析表明，pfSOD mRNA在血细胞和g中大量表达，而在其他测试组织中几乎不表达。用脂多糖（LPS）攻击后，血细胞中pfSOD mRNA的表达增加，在8 h达到最高水平，然后在36 h降至基础水平。这些结果表明，铜/锌超氧化物歧化酶可以用作珍珠牡蛎水生环境污染和细胞压力的生物指标。

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