...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish & Shellfish Immunology >Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica
【24h】

Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica

机译：鳗鳗肝脏中一种由血红蛋白α衍生的新型抗菌肽的分离与鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We isolated and characterized a novel antibacterial peptide, AJHb alpha, derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica. It with concentration of 11.30 mu M exhibited stronger antibacterial activity against pathogenic bacterium 1 x 10(6) cell ml(-1) Edwardsiella tarda than other two bacteria. The extraction procedure for AJHb alpha included extraction with acetate acid, ultrafiltration, cation-exchange chromatography on HiTrap (TM) CM FF, reverse-phase liquid chromatography on Source 5R RPC and 08 RP-HPLC. MALDI-TOF MS suggested that the peptide had an observed molecular weight of 2388.05 Da. Its amino acid sequence determined by Edman degradation was similar to those of hemoglobin alpha chain in other fish by BLAST analysis. A complete N-terminal amino acid sequence of the AJHba was FAHWPDLGPGSPSVKKHGKVIM corresponding to the cDNA sequence by RACE amplification. Its synthetic peptide had strong antibacterial activities against ten Gram-positive or negative bacteria. To our knowledge, AJHba was the first identified fragment of hemoglobin alpha chain with strong antibacterial activity in fish. (C) 2012 Elsevier Ltd. All rights reserved.
机译：我们分离并鉴定了一种新型抗菌肽AJHbα，该肽源于日本鳗鳗肝脏中的血红蛋白α。它的浓度为11.30μM，对其他1个10×6细胞ml（-1）的爱德华氏菌爱德华氏菌显示出比其他两种细菌更强的抗菌活性。 AJHbα的提取程序包括用乙酸提取，超滤，HiTrap（TM）CM FF上的阳离子交换色谱，Source 5R RPC上的反相液相色谱和08 RP-HPLC。 MALDI-TOF MS提示该肽的分子量为2388.05 Da。通过BLAST分析，通过Edman降解确定的氨基酸序列与其他鱼类的血红蛋白α链相似。通过RACE扩增，AJHba的完整N末端氨基酸序列是FAHWPDLGPGSPSVKKHGKVVIM，其对应于cDNA序列。它的合成肽对十种革兰氏阳性或阴性细菌具有很强的抗菌活性。据我们所知，AJHba是最早鉴定出的鱼类中具有强大抗菌活性的血红蛋白α链片段。 （C）2012 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号