• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Fish & Shellfish Immunology >Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis - Part II: Combined effect of temperature and two V-tapetis strains
【24h】

Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis - Part II: Combined effect of temperature and two V-tapetis strains

机译：马尼拉蛤仔弧菌挑战的菲律宾蛤仔中酚氧化酶和超氧化物歧化酶的免疫反应-第二部分：温度和两种V-塔佩特斯菌株的联合作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally infected with two different bacterial strains and challenged with two different temperatures. Bacterial strains used in this study were Vibrio tapetis strain CECT4600(T), the causative agent of Brown Ring Disease (BRD) and V. tapetis strain LP2, supposed less virulent to V. philippinarum. V tapetis is considered to proliferate at low temperatures, i.e. under 21 degrees C. In a global warming context we could hypothesize a decrease of mass mortalities caused by V. tapetis but these thermal changes could also directly impact the immune system of the host V. philippinarum. Thus, the aim of this study was to investigate the effects of the extrapallial injection with V tapetis combined with temperature challenge on two enzymes activities in V. philippinarum. More precisely, after infection, phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immune response, were studied for 30 days in two compartments: the mantle and the hemolymph. Conchyolin Deposit Stages (CDS) and Shell Repair Stages (SRS) were also determined 30 days post-injection as a proxy of the virulence of the tested strains. In this study, we highlighted that host-pathogen interaction in a varying environment affects the enzymatic response of the host. The coupled effect of V. tapetis injection and temperature challenge was detected 30 days post injection and resulted in virulence differences. These findings were supported by CDS and SRS determination in clams and lead to the conclusion that clam's immunity could be enhanced at 22 degrees C while V tapetis virulence is lowered at this temperature. Another result of our study was the increase of PO and SOD basal activities as clams are exposed to warmer temperature. (C) 2015 Elsevier Ltd. All rights reserved.
机译：马尼拉蛤仔，菲律宾的Venerupis philippinarum（Adams和Reeve，1850年）通过实验感染了两种不同的细菌菌株，并用两种不同的温度攻击。在这项研究中使用的细菌菌株是棕带弧菌菌株CECT4600（T），棕环病（BRD）的病原体和V. tapetis菌株LP2，据认为它们对菲律宾弧菌的毒性较小。 V tapetis被认为在低温下（即21摄氏度以下）增殖。在全球变暖的背景下，我们可以推测由V. tapetis引起的质量死亡减少，但这些热变化也可能直接影响宿主V的免疫系统。菲律宾。因此，本研究的目的是研究球囊外注射V tapetis并结合温度刺激对菲律宾葡萄中两种酶活性的影响。更准确地说，在感染后，在两个区室（地幔和血淋巴）中研究了涉及免疫反应的两种主要酶酚氧化酶（PO）和超氧化物歧化酶（SOD）30天。注射后30天还确定了Conchyolin沉积阶段（CDS）和壳修复阶段（SRS），作为测试菌株毒力的替代指标。在这项研究中，我们着重指出在变化的环境中宿主与病原体的相互作用会影响宿主的酶促反应。在注射后30天检测到V. tapetis注射液和温度刺激的耦合作用，并导致毒力差异。这些发现得到蛤CD中CDS和SRS测定的支持，并得出结论，在此温度下蛤lam的免疫力可在22摄氏度下得到增强，而V tapetis毒力则在此温度下降低。我们研究的另一个结果是，随着蛤are暴露于温暖的温度，PO和SOD的基础活性增加。 （C）2015 Elsevier Ltd.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号