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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Physiological characteristics of identified motor units in the mouse extensor digitorum longus muscle: an in vitro approach.
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Physiological characteristics of identified motor units in the mouse extensor digitorum longus muscle: an in vitro approach.

机译:小鼠趾长肌伸肌中确定的运动单位的生理特征:一种体外方法。

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摘要

Physiological, histochemical, and morphometric properties of fast-twitch single motor units were studied in mouse extensor digitorum longus muscles in an in vitro ventral root-nerve-muscle preparation. Single motor units were functionally isolated by microdissection of the ventral root, and the glycogen depletion technique was used to demonstrate the component muscle fibers. Monoclonal antibodies were used to identify their myosin heavy chain composition. The technique allows one to correlate physiological characteristics of single motor units with fiber type but is less useful for morphological assessment of motor unit size as a result of failure to deplete glycogen from all fibers of motor units containing fibers with high oxidative capacity.
机译:在体外腹侧根神经肌肉制剂中,在小鼠指趾长肌中研究了快速抽搐单个运动单位的生理,组织化学和形态计量学特性。通过腹侧根的显微解剖从功能上分离单个运动单位,并使用糖原耗竭技术演示组成性肌纤维。单克隆抗体用于鉴定其肌球蛋白重链组成。该技术允许将单个运动单位的生理特性与纤维类型相关联,但由于未能从包含高氧化能力纤维的运动单位的所有纤维中消耗糖原,因此在运动单位尺寸的形态学评估中不太有用。

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