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首页> 外文期刊>Fish & Shellfish Immunology >Passive protection of shrimp against white spot syndrome virus (WSSV) using specific antibody from egg yolk of chickens immunized with inactivated virus or a WSSV-DNA vaccine
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Passive protection of shrimp against white spot syndrome virus (WSSV) using specific antibody from egg yolk of chickens immunized with inactivated virus or a WSSV-DNA vaccine

机译：使用灭活病毒或WSSV-DNA疫苗免疫的鸡卵黄中的特异性抗体对虾进行白斑综合症病毒（WSSV）的被动保护

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摘要

White spot syndrome virus (WSSV) causes high mortality and large economic losses in cultured shrimp. The VP28, VP19 and VP15 genes encode viral structural proteins of WSSV. In this study, hens were immunized with recombinant plasmid (pCI-VP28/VP19/VP15) with linkers or with inactivated WSSV, which used CpG oligodeoxynucleotides (CpG ODNs) and Freund's adjuvant as adjuvant, respectively. Egg yolk immunoglobulin (IgY) from hens immunized with inactivated vaccine and DNA vaccine was obtained, purified and used for protection of Metapenaeus ensis shrimp against WSSV. The data showed that the antibody response of the hens immunized with the DNA vaccine was improved by CpG ODNs as adjuvant, but was still inferior to inactivated WSSV in both sera and egg yolks. Using specific IgY from hens immunized with inactivated WSSV and DNA vaccine to neutralize WSSV, the challenged shrimp showed 73.3% and 33.3% survival, respectively. Thus, the results suggest that passive immunization strategy with IgY will be a valuable method against WSSV infection in shrimp.
机译：白斑综合症病毒（WSSV）导致养殖虾的高死亡率和巨大的经济损失。 VP28，VP19和VP15基因编码WSSV的病毒结构蛋白。在这项研究中，母鸡用带有接头的重组质粒（pCI-VP28 / VP19 / VP15）或灭活的WSSV免疫，后者分别使用CpG寡脱氧核苷酸（CpG ODN）和弗氏佐剂作为佐剂。获得了用灭活疫苗和DNA疫苗免疫的母鸡的蛋黄免疫球蛋白（IgY），纯化后用于保护Metapenaeus ensis虾抗WSSV。数据显示，用CpG ODNs作为佐剂可改善用DNA疫苗免疫的母鸡的抗体反应，但在血清和蛋黄中均不如灭活的WSSV。用经灭活的WSSV和DNA疫苗免疫的母鸡的特异性IgY中和WSSV，受攻击的虾的存活率分别为73.3％和33.3％。因此，结果表明，用IgY被动免疫策略将是对虾WSSV感染的一种有价值的方法。

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