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首页> 外文期刊>Fish & Shellfish Immunology >The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection
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The major fimbrial subunit protein of Edwardsiella tarda: Vaccine potential, adjuvant effect, and involvement in host infection

机译：爱德华氏菌的主要纤维亚基蛋白：疫苗的潜力，佐剂作用和参与宿主感染

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Edwardsiella tarda is a Gram-negative bacterium that is reckoned one of the most severe fish pathogens. In this study, we analyzed the biological properties of the E. tarda major fimbrial subunit protein, FimA. We found that mutation of fimA resulted in defective biofilm growth, attenuated infectivity against host cells, and impaired ability to disseminate into and colonize host tissues following experimental infection. When used as a subunit vaccine, recombinant FimA (rFimA) elicited a high level of protection in turbot (Scophthalmus maximus) against lethal E. tarda challenge. Immunological analysis showed that rFimA vaccination induced production of specific serum antibodies that bound to live E. tarda via interaction with the FimA on bacterial cells, and that antibody-E. tarda interaction blocked bacterial infection. Furthermore, passive immunization of turbot with anti-rFimA serum before E. tarda infection reduced bacterial loads in fish tissues to significant extents. To examine the adjuvant potential of FimA, turbot were vaccinated with rVhhP2, a protective Vibrio harveyi antigen, in the presence or absence of rFimA. Subsequent analysis showed that the presence of rFimA significantly augmented the protectivity of rVhhP2. ELISA and quantitative real time RT-PCR showed that rFimA significantly increased rVhhP2-specific serum antibody production and enhanced the expression of immune relevant genes. Taken together, these results indicate that FimA is a virulence-associated protein that possesses vaccine as well as adjuvant potentials, and that the immunoprotectivity of FimA is most likely due to its ability to induce specific immune response that inhibits E. tarda infection. (C) 2013 Elsevier Ltd. All rights reserved.
机译：泰氏爱德华氏菌是革兰氏阴性细菌，被认为是最严重的鱼类病原体之一。在这项研究中，我们分析了大肠埃希氏菌主要纤维亚单位蛋白FimA的生物学特性。我们发现，fimA突变导致缺陷的生物膜生长，减弱了对宿主细胞的感染力，并削弱了实验性感染后向宿主组织内扩散和定殖的能力。当用作亚单位疫苗时，重组FimA（rFimA）在大菱t（Scophthalmus maximus）中引起了针对致死性大肠杆菌的挑战。免疫学分析表明，rFimA疫苗接种可诱导特异性血清抗体的产生，该抗体通过与细菌细胞上的FimA相互作用而与活的塔氏大肠杆菌结合，并产生该抗体-E。 tarda相互作用阻止细菌感染。此外，在塔氏大肠杆菌感染之前用抗rFimA血清被动免疫大菱t，可以大大减少鱼组织中的细菌负荷。为了检查FimA的佐剂潜力，在存在或不存在rFimA的情况下，用保护性哈维弧菌抗原rVhhP2对大菱t进行疫苗接种。随后的分析表明，rFimA的存在显着增强了rVhhP2的保护性。 ELISA和实时定量RT-PCR表明，rFimA显着增加了rVhhP2特异性血清抗体的产生，并增强了免疫相关基因的表达。综上所述，这些结果表明，FimA是一种具有毒力的蛋白质，具有疫苗和佐剂的潜力，而FimA的免疫保护性极有可能是由于其诱导抑制塔氏大肠杆菌感染的特异性免疫反应的能力。 （C）2013 Elsevier Ltd.保留所有权利。

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