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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Effects of sodium fluoride on the mechanical activity in mouse gastric preparations.
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Effects of sodium fluoride on the mechanical activity in mouse gastric preparations.

机译：氟化钠对小鼠胃内制剂机械活性的影响。

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摘要

The aim of the present study was to investigate the responses induced by sodium fluoride (NaF) on gastric mechanical activity, using mouse whole-stomach preparations. The mechanical activity was recorded in vitro as changes of intraluminal pressure. In most of the preparations, NaF induced a tetrodotoxin-insensitive biphasic effect characterized by early relaxation followed by slowly developing contractile response. The contraction was dependent on the concentration of NaF, whereas the relaxation was observed at only 10-30 mmol/L NaF. The contractile effect was significantly reduced by nifedipine (an L-type Ca(2+) channel blocker), ryanodine or ruthenium red (inhibitors of Ca(2+) release from sarcoplasmic reticulum), and GF109203X (a protein kinase C inhibitor). Moreover, it was abolished by neomycin (an inhibitor of phospholipase C) and potentiated by SQ22536 (an inhibitor of adenylyl cyclase). All the drugs significantly increased the relaxation, except SQ22536, which abolished it. The present results suggest that NaF causes a complex mechanical response in the whole-stomach, which might explain gastric discomfort after fluoride ingestion. The relaxation appears owing to production of cAMP, while the contractile effects imply activation of phospholipase C, protein kinase C, influx of Ca(2+), and release of Ca(2+) from ryanodine-sensitive intracellular store.
机译：本研究的目的是使用小鼠全胃制剂研究氟化钠（NaF）诱导的对胃机械活动的反应。在体外将机械活性记录为腔内压力的变化。在大多数制剂中，NaF诱导了对河豚毒素不敏感的双相效应，其特征是早期松弛，然后缓慢发展收缩反应。收缩取决于NaF的浓度，而在仅10-30mmol / L的NaF下观察到松弛。硝苯地平（一种L型Ca（2+）通道阻滞剂），ryanodine或钌红（从肌质网释放的Ca（2+）抑制剂）和GF109203X（一种蛋白激酶C抑制剂）显着降低了收缩作用。此外，它被新霉素（磷脂酶C的抑制剂）废除，并被SQ22536（腺苷酸环化酶的抑制剂）增强。除SQ22536消除了松弛作用外，所有药物均显着增加了松弛作用。目前的结果表明，NaF在整个胃中引起复杂的机械反应，这可能解释了氟化物摄入后的胃部不适。松弛是由于cAMP的产生而引起的，而收缩效应则暗示着磷脂酶C，蛋白激酶C的激活，Ca（2+）的涌入以及从Ryanodine敏感的细胞内存储中释放Ca（2+）。

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