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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Urinary protein profiling with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry in ETB receptor-deficient rats.
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Urinary protein profiling with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry in ETB receptor-deficient rats.

机译：ETB受体缺乏大鼠的表面增强激光解吸/电离飞行时间质谱分析尿蛋白谱。

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The pathways leading to salt-sensitive hypertension and renal damage in rescued ETB receptor-deficient (ETBRd) rats are still unknown. The objective of the study was therefore to identify modifications of urinary peptide and protein expression in ETBRd rats (n = 9) and wild-type controls (n = 6) using SDS - polyacrylamide gel electrophoresis (SDS-PAGE) and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology. Glomerular filtration rate, glomerulosclerosis, and tubulointerstitial fibrosis did not differ between the groups. ETBRd rats showed slightly higher blood pressure (p < 0.001), media/lumen ratio of intrarenal arteries (p < 0.01), and albuminuria (p < 0.01). SDS-PAGE confirmed albuminuria, but showed no differences in the urinary excretion of low molecular weight proteins (<60 kDa). SELDI-TOF-MS profiling revealed 9 proteomic features at molecular masses (Da) of 2720, 2980, 3130, 3345, 6466, 6682, 8550, 18 729, and 37 492, which were significantly elevated (p < 0.02) in urine of ETBRd rats. The results demonstrate that, independent of structural changes in the kidneys, ETB-receptor deficiency causes specific differences in urinary peptide and protein excretion. SELDI-TOF-MS may be a valuable tool for the characterization of urinary biomarkers helping to uncover the mechanism of ETBR action in the kidney.
机译：在拯救的ETB受体缺乏（ETBRd）大鼠中导致盐敏感性高血压和肾损害的途径仍然未知。因此，本研究的目的是使用SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）和表面增强激光鉴定ETBRd大鼠（n = 9）和野生型对照（n = 6）中尿肽和蛋白质表达的修饰解吸/电离飞行时间质谱（SELDI-TOF-MS）技术。两组之间的肾小球滤过率，肾小球硬化和肾小管间质纤维化无差异。 ETBRd大鼠显示出略高的血压（p <0.001），肾内动脉的中/腔比（p <0.01）和白蛋白尿（p <0.01）。 SDS-PAGE证实为蛋白尿，但低分子量蛋白（<60 kDa）的尿排泄无差异。 SELDI-TOF-MS分析显示9个蛋白质组学特征的分子质量（Da）为2720、2980、3130、3345、6466、6682、8550、18 729和37492，在尿液中尿中的这些蛋白质组显着升高（p <0.02） ETBRd大鼠。结果表明，与肾脏的结构变化无关，ETB受体缺乏引起尿肽和蛋白质排泄的特异性差异。 SELDI-TOF-MS可能是表征尿液生物标志物的有价值的工具，有助于揭示肾脏中ETBR作用的机制。

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