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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Effect of uroguanylin on potassium and bicarbonate transport in rat renal tubules.
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Effect of uroguanylin on potassium and bicarbonate transport in rat renal tubules.

机译：尿鸟苷对大鼠肾小管中钾和碳酸氢根转运的影响。

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The effect of uroguanylin (UGN) on K+ and H+ secretion in the renal tubules of the rat kidney was studied using in vivo stationary microperfusion. For the study of K+ secretion, a tubule was punctured to inject a column of FDC-green-colored Ringer's solution with 0.5 mmol KCl/L +/- 10-6 mol UGN/L, and oil was used to block fluid flow. K+ activity and transepithelial potential differences (PD) were measured with double microelectrodes (K+ ion-selective resin vs. reference) in the distal tubules of the same nephron. During perfusion, K+ activity rose exponentially, from 0.5 mmol/L to stationary concentration, allowing for the calculation of K+ secretion (JK). JK increased from 0.63 +/- 0.06 nmol.cm-2.s-1 in the control group to 0.85 +/- 0.06 in the UGN group (p < 0.01). PD was -51.0 +/- 5.3 mV in the control group and -50.3 +/- 4.98 mV in the UGN group. In the presence of 10-7 mol iberiotoxin/L, the UGN effect was abolished: JK was 0.37 +/- 0.038 nmol.cm-2.s-1 in the absence of, and 0.38 +/- 0.025 in thepresence of, UGN, indicating its action on maxi-K channels. In another series of experiments, renal tubule acidification was studied, using a similar method: proximal and distal tubules were perfused with solutions containing 25 mmol NaHCO3/L. Acidification half-time was increased both in proximal and distal segments and, as a consequence, bicarbonate reabsorption decreased in the presence of UGN (in proximal tubules, from 2.40 +/- 0.26 to 1.56 +/- 0.21 nmol.cm-2.s-1). When the Na+/H+ exchanger was inhibited by 10-4 mol hexamethylene amiloride (HMA)/L, the control and UGN groups were not significantly different. In the late distal tubule, after HMA, UGN significantly reduced JHCO3-, indicating an effect of UGN on H+-ATPase. These data show that UGN stimulated JK+ by acting on maxi-K channels, and decreased JHCO3- by acting on NHE3 in proximal and H+-ATPase in distal tubules.
机译：使用体内固定微灌流研究了尿鸟苷素（UGN）对大鼠肾脏肾小管中K +和H +分泌的影响。为了研究K +分泌，穿刺一根小管以注入FDC绿色林格氏溶液的色谱柱，其中加入0.5 mmol KCl / L +/- 10-6 mol UGN / L，并使用油阻止流体流动。在同一肾单位的远端小管中，用双微电极（K +离子选择性树脂与参比）测量K +活性和跨上皮电位差（PD）。在灌注过程中，K +活性从0.5 mmol / L呈指数级上升至固定浓度，从而可以计算K +分泌（JK）。 JK从对照组的0.63 +/- 0.06 nmol.cm-2.s-1增加到UGN组的0.85 +/- 0.06（p <0.01）。对照组的PD为-51.0 +/- 5.3 mV，而UGN组的PD为-50.3 +/- 4.98 mV。在存在10-7 mol iberio毒素/ L的情况下，取消了UGN的作用：在不存在UGN的情况下，JK为0.37 +/- 0.038 nmol.cm-2.s-1;在存在UGN的情况下，JK为0.38 +/- 0.025 ，指示其对maxi-K通道的作用。在另一系列实验中，使用相似的方法研究了肾小管的酸化：用含25 mmol NaHCO3 / L的溶液灌注近端和远端小管。在近端和远端段酸化的半衰期均增加，结果，在存在UGN的情况下，碳酸氢盐的重吸收减少（在近端小管中，从2.40 +/- 0.26降至1.56 +/- 0.21 nmol.cm-2.s -1）。当Na + / H +交换剂被10-4 mol六亚甲基阿米洛利（HMA）/ L抑制时，对照组和UGN组没有显着差异。在HMA之后，在远端末梢小管中，UGN显着降低了JHCO3-，表明UGN对H + -ATPase有影响。这些数据表明，UGN通过作用于maxi-K通道刺激JK +，并通过作用于近端的NHE3和远端小管的H + -ATPase降低JHCO3-。

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