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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Differences between femoral artery and vein smooth muscle cells in volume-regulated chloride channels
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Differences between femoral artery and vein smooth muscle cells in volume-regulated chloride channels

机译:氯调节通道中股动脉和静脉平滑肌细胞之间的差异

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摘要

The purpose of the present study was to compare the differences between the role of volume-regulated Cl- channels (VRCCs) in veins and arteries. We used the whole cell patch clamp and fluorescence imaging techniques to evaluate swellinginduced Cl- current (ICl,vol) and changes in the intracellular concentrations of Cl- ([Cl-]i) induced by hypotonic solutions in rat femoral artery cells (FASMCs) and vein smooth muscle cells (FVSMCs). ICl,vol and [Cl-]i decline induced by hypotonic solution were more prominent in FASMCs than in FVSMCs. ICl,vol and the alterations in [Cl-]i were gradually increased as the number of cell passages increased. However, the regulatory function of tyrosine protein phosphorylation in volume-regulated chloride movement is prominent in veins. The expression of ClC-3 was higher in FASMCs than in FVSMCs. VRCC activity is more pronounced in rat femoral arteries than in veins. VRCC activity and tyrosine protein phosphorylation regulative function increase gradually as vascular cells switch from contractile to proliferative phenotypes.
机译:本研究的目的是比较在静脉和动脉中容积调节的Cl-通道(VRCC)的作用之间的差异。我们使用全细胞膜片钳和荧光成像技术评估大鼠股动脉细胞(FASMCs)的低渗溶液诱导的肿胀诱导的Cl-电流(ICl,vol)和细胞内Cl-([Cl-] i)浓度的变化。和静脉平滑肌细胞(FVSMC)。由低渗溶液引起的ICl,vol和[Cl-] i下降在FASMC中比在FVSMC中更为明显。随着细胞传代次数的增加,ICl,vol和[Cl-] i的变化逐渐增加。然而,酪氨酸蛋白磷酸化在调节氯化物运动中的调节功能在静脉中很明显。在FASMC中,ClC-3的表达高于在FVSMC中。在大鼠股动脉中,VRCC活动比在静脉中更为明显。随着血管细胞从收缩型向增生型转变,VRCC活性和酪氨酸蛋白磷酸化调节功能逐渐增强。

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