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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Ageing-induced decrease in cardiac mitochondrial function in healthy rats
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Ageing-induced decrease in cardiac mitochondrial function in healthy rats

机译：衰老导致健康大鼠心脏线粒体功能下降

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摘要

It is widely recognized that mitochondrial dysfunction is a key component of the multifactorial process of ageing. The effects of age on individual components of mitochondrial function vary across species and strains. In this study we investigated the oxygen consumption, the mitochondrial membrane potential (ΔΨ), the sensitivity of mitochondrial permeability transition pore (mPTP) to calcium overload, and the production of reactive oxygen species (ROS) in heart mitochondria isolated from old compared with adult healthy Sprague-Dawley rats. Respirometry studies and ΔΨ measurements were performed with an Oxygraph-2k equipped with a tetraphenylphosphonium electrode. ROS production and calcium retention capacity were measured spectrofluorimetrically. Our results show an important decline for all bioenergetic parameters for both complex I and complex II supported-respiration, a decreased ΔΨ in mitochondria energized with complex I substrates, and an increased mitochondrial ROS production in the old compared with the adult group. Mitochondrial sensitivity to Ca2+-induced mPTP opening was also increased in the old compared with the adult animals. Moreover, the protective effect of cyclosporine A on mPTP opening was significantly reduced in the old group. We conclude that healthy ageing is associated with a decrease in heart mitochondria function in Sprague-Dawley rats.
机译：众所周知，线粒体功能障碍是衰老的多因素过程的关键组成部分。年龄对线粒体功能各个组成部分的影响因物种和品系而异。在这项研究中，我们调查了老年人和成年人分离的心脏线粒体中的耗氧量，线粒体膜电位（ΔΨ），线粒体通透性过渡孔（mPTP）对钙超载的敏感性以及活性氧物质（ROS）的产生健康的Sprague-Dawley大鼠。用配备有四苯基phosph电极的Oxygraph-2k进行呼吸测定研究和ΔΨ测量。用荧光分光光度法测定ROS的产生和钙保留能力。我们的结果表明，与成人组相比，复杂I和复杂II支持的呼吸的所有生物能参数均显着下降，由复杂I底物激发的线粒体ΔΨ降低，并且线粒体ROS产量增加。与成年动物相比，线粒体对Ca2 +诱导的mPTP开放的敏感性也有所提高。而且，老年组环孢素A对mPTP开放的保护作用明显降低。我们得出结论，健康的衰老与Sprague-Dawley大鼠心脏线粒体功能的降低有关。

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