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首页> 外文期刊>Forestry >Preparing for the invasion: efficacy of DNA barcoding to discern the host range of myrtle rust (Puccinia psidii) among species of Myrtaceae
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Preparing for the invasion: efficacy of DNA barcoding to discern the host range of myrtle rust (Puccinia psidii) among species of Myrtaceae

机译：为入侵做准备：DNA条形码识别桃金娘科物种间紫金锈病（Puccinia psidii）寄主范围的功效

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摘要

Puccinia psidii is a major threat to members of the Myrtaceae worldwide. New Zealand is still free of P. psidii but possesses significant Eucalyptus plantations as well as valuable native tree species such as Metrosideros excelsa and Leptospermum scoparium that could be at risk in the event of an incursion. As part of preparing for a potential incursion, we generated a barcode reference Library for >100 diverse species of Myrtaceae occurring in New Zealand by sequencing internal transcribed spacer (ITS), external transcribed spacer (ETS) and maturase K (matK), or by obtaining relevant sequences from GenBank. The Myrtaceae DNA barcoding database will enable rapid identification of Large numbers of host species in the event of a myrtle rust incursion. We undertook a comparative analysis of the ability for the three mentioned Loci to discriminate species. Interspecific divergence was assessed by mean interspecific distance, theta prime and minimum interspecific distance; intraspecific variation was evaluated by mean intraspecific difference, theta and coalescent depth. Overall identification efficiency of the three Loci sequenced was determined using BLAST1 and Near Distance methods. Barcoding gaps between inter and intraspecific divergences were also analysed. ITS and ETS share similar mean interspecific distance, theta prime and minimum interspecific distance values - both higher than obtained for matK. In contrast, in terms of intraspecific variation, matK had higher values than ITS and ETS in all three metrics analysed. ITS had the highest identification success rate for species followed by matK and ETS, respectively, as measured by BLAST1 and Near Distance methods. Overall identification success rate increases when a combination of ITS and matK in particular is used. The sequence data are not only a valuable reference collection for a myrtle rust response but also a national botanical resource.
机译：psiuccini psidii是对全世界桃金娘科成员的主要威胁。新西兰仍然没有P. psidii，但拥有大量的桉树人工林以及珍贵的本地树种，例如Metrosideros excelsa和Leptospermum scoparium，如果受到入侵，可能会处于危险之中。作为准备潜在入侵的一部分，我们通过对内部转录间隔区（ITS），外部转录间隔区（ETS）和成熟酶K（matK）进行测序，或者通过对从GenBank获得相关序列。桃金娘科的DNA条形码数据库将在紫金娘锈病入侵的情况下快速识别大量宿主物种。我们对上述三个基因座区分物种的能力进行了比较分析。种间差异通过平均种间距离，θ素数和最小种间距离进行评估;通过平均种内差异，θ和聚结深度评估种内变异。使用BLAST1和近距离方法确定了三个已测序基因座的总体识别效率。还分析了种间和种内差异之间的条形码差异。 ITS和ETS具有相似的平均种间距离，θ素数和最小种间距离值-均高于matK的值。相比之下，就种内变异而言，在所分析的所有三个指标中，matK的值均高于ITS和ETS。通过BLAST1和近距离方法测量，ITS的物种识别成功率最高，其次是matK和ETS。特别是将ITS和matK结合使用时，总体识别成功率会提高。序列数据不仅是一种针对桃金娘锈反应的有价值的参考资料，而且还是一种国家植物资源。

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