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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Anabolic responsiveness of skeletal muscles correlates with androgen receptor protein but not mRNA.
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Anabolic responsiveness of skeletal muscles correlates with androgen receptor protein but not mRNA.

机译：骨骼肌的合成代谢反应与雄激素受体蛋白有关，与mRNA无关。

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摘要

Anabolic effects of androgens on skeletal muscle are well documented, but the physiological and biochemical bases of these effects are poorly understood. Skeletal muscles that differ in their androgen responsiveness can be used to examine these mechanisms. We compared androgen receptor mRNA and protein levels of the rat levator ani, a perineal skeletal muscle that depends on androgens for its normal maintenance and function with that of the rat extensor digitorum longus, a limb muscle that does not require androgens. Western immunoblotting indicated that androgen receptor protein is significantly elevated in the levator ani relative to the extensor digitorum longus. Surprisingly, steady state androgen receptor mRNA levels were equivalent in these muscles, as determined by Northern blot analysis and quantitative RT-PCR. These results suggest that androgen responsiveness of skeletal muscles is determined by the level of androgen receptor protein in a particular muscle and that androgen receptor protein content is regulated by translational or post-translational mechanisms.
机译：雄激素对骨骼肌的合成代谢作用已有充分文献记载，但对这些作用的生理和生化基础知之甚少。雄激素反应性不同的骨骼肌可用于检查这些机制。我们比较了大鼠肛提肌ani的雄激素受体mRNA和蛋白质水平，aniper是正常的维持和功能依赖于会阴激素的会阴骨骼肌，与不需要趾甲的四肢伸指长肌。 Western免疫印迹表明，相对于趾长伸肌，雄性激素受体蛋白在肛提肌中明显升高。出乎意料的是，通过Northern印迹分析和定量RT-PCR确定，这些肌肉中的稳态雄激素受体mRNA水平是相等的。这些结果表明，骨骼肌的雄激素反应性取决于特定肌肉中雄激素受体蛋白的水平，并且雄激素受体蛋白的含量受翻译或翻译后机制的调节。

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