...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Stretch induced accumulation of total Ca and Na in cytosol and nucleus: a comparison between cardiac trabeculae and isolated myocytes.
【24h】

Stretch induced accumulation of total Ca and Na in cytosol and nucleus: a comparison between cardiac trabeculae and isolated myocytes.

机译：拉伸诱导胞质和细胞核中总钙和钠的积累：心脏小梁细胞与分离的心肌细胞之间的比较。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By means of electron probe microanalysis (EPMA), we quantified changes in total sodium [Na] and calcium [Ca] concentration owing to the following: (i) local axial stretch (LAS) of isolated rat myocytes and (ii) end-to-end stretch (ETES) of rat ventricular trabeculae. For LAS, the distance between patch pipette and a cell-attached stylus was increased by maximally 20%; this activated a nonselective cationic current I(SAC) of approximately -0.5 nA, which was blocked by streptomycin. Trabeculae were stretched end-to-end from 85% L(max) to L(max). Stretch increased cytosolic [Na](total) by 34% in isolated myocytes (p < 0.001) and by 43% in trabeculae (p < 0.001). The increment in nuclear [Na](total) was 21% in myocytes (p < 0.01) and 20% in trabeculae (p < 0.001). Stretch increased [Ca](total) in isolated myocytes, in both cytosol (from 0.63 +/- 0.09 to 1.09 +/- 0.20 mmol/L, p < 0.05) and nucleus (from 0.33 +/- 0.05 to 0.64 +/- 0.13 mmol/L, p < 0.05). In trabeculae, the stretch-induced increment of 51% in cytosolic [Ca](total) remained nonsignificant (p < 0.15). In the nucleus, [Ca](total) did not change. We interpret the difference of stretch on nuclear calcium in myocytes vs. trabeculae with the assumption that LAS, but not ETES, produces shear-stress components that translate the mechanical stimulus deeply into the cell where it may modulate [Ca](total) by signals independent of I(SAC).
机译：通过电子探针显微分析（EPMA），我们量化了由于以下原因引起的总钠[Na]和钙[Ca]浓度的变化：（i）离体大鼠心肌细胞的局部轴向拉伸（LAS）和（ii）端到端室小梁末端伸展（ETES）。对于LAS，贴片移液器和附有细胞的测针之间的距离最大增加了20％。这激活了约-0.5 nA的非选择性阳离子电流I（SAC），该电流被链霉素阻止。将小梁从85％L（max）端到端拉伸到L（max）。拉伸使离体心肌细胞的胞质[Na]（总）增加34％（p <0.001），而小梁细胞增加43％（p <0.001）。心肌细胞中核[Na]（总量）的增加为21％（p <0.01），小梁细胞为20％（p <0.001）。在细胞溶胶（从0.63 +/- 0.09到1.09 +/- 0.20 mmol / L，p <0.05）和细胞核（从0.33 +/- 0.05到0.64 +/-）中，拉伸都增加了分离的心肌细胞的[Ca]（总） 0.13 mmol / L，p <0.05）。在小梁中，拉伸诱导的胞质[Ca]（总量）中51％的增加保持不显着（p <0.15）。在细胞核中，[Ca]（总数）没有变化。我们假设LAS（而非ETES）会产生剪切应力分量，从而将机械刺激深深地转化为细胞，在信号作用下可以调节[Ca]（总），从而解释了肌细胞与小梁细胞中钙离子舒张的差异。独立于I（SAC）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号